|PROSITE documentation PDOC51140|
The Coupling of Ubiquitin conjugation to ER degradation or CUE domain is a ~44 residue ubiquitin binding domain, present in eukaryotic proteins of trafficking and ubiquitination pathways. Ubiquitin is a 76 amino acid polypeptide that can be attached to proteins to alter their activity or location (see <PDOC00271>). Monoubiquitination and the addition of polyubiquitin chains are important cellular regulatory signals. The CUE domain was named after baker's yeast Cue1 protein . Some domains which can occur in combination with the CUE domain are the RING-type zinc finger (see <PDOC00449>), the Smr domain (see <PDOC50828>) or the C2 domain (see <PDOC00380>). CUE domains can bind monoubiquitin and can be required for ubiquitination of the protein in which they are found [2,3,4].
The 3D structure of the CUE domain comprises three α helices arranged in a bundle (see <PDB:1OTR>), similar to the fold of the UBA domain (see <PDOC50030>). The binding surface is a hydrophobic patch that is complementary to the ubiquitin hydrophobic surface. The CUE domain contains two conserved motifs that bind ubiquitin, i.e. (MF)P, C-terminal to the first helix, and a di-leucine-like motif in the third helix.
Some proteins known to contain a CUE domain:
The profile we developed covers the entire CUE domain.Last update:
July 2005 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|Title||Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction.|
|Source||Biochem. J. 351:527-535(2000).|
|2||Authors||Shih S.C. Prag G. Francis S.A. Sutanto M.A. Hurley J.H. Hicke L.|
|Title||A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain.|
|Source||EMBO J. 22:1273-1281(2003).|
|3||Authors||Prag G. Misra S. Jones E.A. Ghirlando R. Davies B.A. Horazdovsky B.F. Hurley J.H.|
|Title||Mechanism of ubiquitin recognition by the CUE domain of Vps9p.|
|4||Authors||Kang R.S. Daniels C.M. Francis S.A. Shih S.C. Salerno W.J. Hicke L. Radhakrishnan I.|
|Title||Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding.|