PROSITE documentation PDOC51265Zinc finger Dbf4-type profile
In eukaryotes, initiation of DNA replication requires the assembly of pre-replication complexes (pre-RCs) on chromatin during the G1 phase. In the S phase, pre-RCs are activated by two protein kinases, Cdk2 and Cdc7, which results in the loading of replication factors and the unwinding of replication origins by the MCM helicase complex [1]. Cdc7 is a serine/threonine kinase (see <PDOC00100>)that is conserved from yeast to human. It is regulated by its association with a regulatory subunit, the Dbf4 protein. This complex is often referred to as DDK (Dbf4-dependent kinase) [2].
DBF4 contains an N-terminal BRCT domain (see <PDOC50172>) and a C-terminal conserved region that could potentially coordinate one zinc atom, the DBF4-type zinc finger. The zinc finger is important for the interaction with Cdc7 [3,4].
The profile we developed covers the entire DBF4-type zinc finger.
Last update:November 2006 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Nasmyth K. |
| Title | Viewpoint: putting the cell cycle in order. | |
| Source | Science 274:1643-1645(1996). | |
| PubMed ID | 8984634 |
| 2 | Authors | Duncker B.P. Brown G.W. |
| Title | Cdc7 kinases (DDKs) and checkpoint responses: lessons from two yeasts. | |
| Source | Mutat. Res. 532:21-27(2003). | |
| PubMed ID | 14643426 |
| 3 | Authors | Hardy C.F. Pautz A. |
| Title | A novel role for Cdc5p in DNA replication. | |
| Source | Mol. Cell. Biol. 16:6775-6782(1996). | |
| PubMed ID | 8943332 |
| 4 | Authors | Dowell S.J. Romanowski P. Diffley J.F. |
| Title | Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo. | |
| Source | Science 265:1243-1246(1994). | |
| PubMed ID | 8066465 |
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