PROSITE documentation PDOC51265
Zinc finger Dbf4-type profile


In eukaryotes, initiation of DNA replication requires the assembly of pre-replication complexes (pre-RCs) on chromatin during the G1 phase. In the S phase, pre-RCs are activated by two protein kinases, Cdk2 and Cdc7, which results in the loading of replication factors and the unwinding of replication origins by the MCM helicase complex [1]. Cdc7 is a serine/threonine kinase (see <PDOC00100>)that is conserved from yeast to human. It is regulated by its association with a regulatory subunit, the Dbf4 protein. This complex is often referred to as DDK (Dbf4-dependent kinase) [2].

DBF4 contains an N-terminal BRCT domain (see <PDOC50172>) and a C-terminal conserved region that could potentially coordinate one zinc atom, the DBF4-type zinc finger. The zinc finger is important for the interaction with Cdc7 [3,4].

The profile we developed covers the entire DBF4-type zinc finger.

Last update:

November 2006 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

ZF_DBF4, PS51265; Zinc finger DBF4-type profile  (MATRIX)


1AuthorsNasmyth K.
TitleViewpoint: putting the cell cycle in order.
SourceScience 274:1643-1645(1996).
PubMed ID8984634

2AuthorsDuncker B.P. Brown G.W.
TitleCdc7 kinases (DDKs) and checkpoint responses: lessons from two yeasts.
SourceMutat. Res. 532:21-27(2003).
PubMed ID14643426

3AuthorsHardy C.F. Pautz A.
TitleA novel role for Cdc5p in DNA replication.
SourceMol. Cell. Biol. 16:6775-6782(1996).
PubMed ID8943332

4AuthorsDowell S.J. Romanowski P. Diffley J.F.
TitleInteraction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo.
SourceScience 265:1243-1246(1994).
PubMed ID8066465

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