PROSITE documentation PDOC51299
APSES-type HTH DNA-binding domain profile


The APSES (ASM-1, Phd1, StuA, EFG1, and Sok2) domain is an ~110-residue sequence-specific DNA-binding domain found in a family of fungal transcription factors that regulate development or cell cycle progression. The APSES domain is found associated with ankyrin repeats (see <PDOC50088>) and a heterodimerization domain [1,2].

The APSES domain consists of a six-stranded β-sheet segment folded against two pairs of α-helices in the topology of the winged helix-turn-helix (HTH) family of proteins (see <PDB:1L3G>). The αA/αB helical pair corresponds to the HTH motif [3,4,5].

Some proteins known to contain an APSES domain are listed below:

  • Saccharomyces cerevisiae Mlu-1 box binding protein (MBP1), a cell cycle transcription factor.
  • Schizosaccharomyces pombe cell division cycle-related proteins res1/sct1 and res2/pct1, which are homologous to MBP1.
  • Schizosaccharomyces pombe start control protein cdc10.
  • Emericella nidulans (Aspergillus nidulans) cell pattern formation- associated protein stuA. It regulates the transformation of undifferentiated hyphal elements into a complex multicellular structure.
  • Saccharomyces cerevisiae transcription factor PHD1.
  • Saccharomyces cerevisiae protein SOK2. It represses the transition from unicellular growth to pseudohyphae.
  • Candida albicans enhanced filamentous growth protein (EFG1).
  • Neurospora crassa ascospore maturation 1 protein (Asm-1). It has a role in spore maturation.

The profile we developed covers the entire APSES domain.

Last update:

March 2007 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

HTH_APSES, PS51299; APSES-type HTH DNA-binding domain profile  (MATRIX)


1AuthorsAramayo R. Peleg Y. Addison R. Metzenberg R.
TitleAsm-1+, a Neurospora crassa gene related to transcriptional regulators of fungal development.
SourceGenetics 144:991-1003(1996).
PubMed ID8913744

2AuthorsDutton J.R. Johns S. Miller B.L.
TitleStuAp is a sequence-specific transcription factor that regulates developmental complexity in Aspergillus nidulans.
SourceEMBO J. 16:5710-5721(1997).
PubMed ID9312029

3AuthorsXu R.-M. Koch C. Liu Y. Horton J.R. Knapp D. Nasmyth K. Cheng X.
TitleCrystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis.
SourceStructure 5:349-358(1997).
PubMed ID9083114

4AuthorsTaylor I.A. Treiber M.K. Olivi L. Smerdon S.J.
TitleThe X-ray structure of the DNA-binding domain from the Saccharomyces cerevisiae cell-cycle transcription factor Mbp1 at 2.1 A resolution.
SourceJ. Mol. Biol. 272:1-8(1997).
PubMed ID9299332

5AuthorsNair M. McIntosh P.B. Frenkiel T.A. Kelly G. Taylor I.A. Smerdon S.J. Lane A.N.
TitleNMR structure of the DNA-binding domain of the cell cycle protein Mbp1 from Saccharomyces cerevisiae.
SourceBiochemistry 42:1266-1273(2003).
PubMed ID12564929

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