PROSITE logo
Black ribbon
We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE documentation PDOC51532
PITH domain profile


View entry in original PROSITE document format
View entry in raw text format (no links)
PURL: https://purl.expasy.org/prosite/documentation/PDOC51532

Description

The proteasome-interacting thioredoxin (PITH) domain is a general proteasome-interacting module [1].

The PITH domain is dominated by a jelly roll β-sandwich structure (see <PDB:1XOY>). The β-sandwich is formed by face-to-face packing of two anti-parallel β-sheets. Another two-stranded β-sheet seals off one end of the β-barrel [2,3].

Some proteins known to contain a PITH domain are listed below:

  • Human Txnl1 or TRP32, a thioredoxin-like protein implicated in endocytosis and in protection against glucose deprivation-induced cytotoxicity.
  • Human C1ORF28, an uncharacterized protein that does not contain any other known protein domains, but has been found to associate with 26S proteasomes.
  • Arabidopsis thaliana At3g04780, a single domain protein.

The profile we developed covers the entire PITH domain.

Last update:

March 2011 / First entry.

-------------------------------------------------------------------------------


Technical section

PROSITE method (with tools and information) covered by this documentation:

PITH, PS51532; PITH domain profile  (MATRIX)


References

1AuthorsAndersen K.M. Madsen L. Prag S. Johnsen A.H. Semple C.A. Hendil K.B. Hartmann-Petersen R.
TitleThioredoxin Txnl1/TRP32 is a redox-active cofactor of the 26 S proteasome.
SourceJ. Biol. Chem. 284:15246-15254(2009).
PubMed ID19349277
DOI10.1074/jbc.M900016200

2AuthorsSong J. Tyler R.C. Wrobel R.L. Frederick R.O. Vojtek F.C. Jeon W.B. Lee M.S. Markley J.L.
TitleSolution structure of At3g04780.1-des15, an Arabidopsis thaliana ortholog of the C-terminal domain of human thioredoxin-like protein.
SourceProtein Sci. 14:1059-1063(2005).
PubMed ID15741346
DOI10.1110/ps.041246805

3AuthorsGoroncy A.K. Koshiba S. Tochio N. Tomizawa T. Inoue M. Tanaka A. Sugano S. Kigawa T. Yokoyama S.
TitleSolution structure of the C-terminal DUF1000 domain of the human thioredoxin-like 1 protein.
SourceProteins 78:2176-2180(2010).
PubMed ID20455272
DOI10.1002/prot.22719



PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.