PROSITE documentation PDOC51542
FYR domain FYRN and FYRC motif profiles


The FYRN and FYRC sequence motifs are two poorly characterized phenylalanine/ tyrosine-rich regions of around 50 and 100 amino acids, respectively, that are found in a variety of chromatin-associated proteins [1,2,3,4]. They are particularly common in histone H3K4 methyltransferases most notably in a family of proteins that includes human mixed lineage leukemia (MLL) and the Drosophila melanogaster protein trithorax. Both of these enzymes play a key role in the epigenetic regulation of gene expression during development, and the gene coding for MLL is frequently rearranged in infant and secondary therapy-related acute leukemias. They are also found in transforming growth factor β regulator 1 (TBRG1), a growth inhibitory protein induced in cells undergoing arrest in response to DNA damage and transforming growth factor (TGF)-β1. As TBRG1 has been shown to bind to both the tumor suppressor p14ARF and MDM2, a key regulator of p53, it is also known as nuclear interactor of ARF and MDM2 (NIAM). In most proteins, the FYRN and FYRC regions are closely juxtaposed, however, in MLL and its homologues they are far distant. To be fully active, MLL must be proteolytically processed by taspase1, which cleaves the protein between the FYRN and FYRC regions [5]. The N-terminal and C-terminal fragments remain associated after proteolysis apparently as a result of an interaction between the FYRN and FYRC regions. How proteolytic processing regulates the activity of MLL is not known. Intriguingly, the FYRN and FYRC motifs of a second family of histone H3K4 methyltransferases, represented by MLL2 and MLL4 in humans and TRR in Drosophila melanogaster, are closely juxtaposed. FYRN and FYRC motifs are found in association with modules that create or recognize histone modifications in proteins from a wide range of eukaryotes, and it is likely that in these proteins they have a conserved role related to some aspect of chromatin biology [4].

The FYRN and FYRC regions are not separate independently folded domains, but are components of a distinct protein module, The FYRN and FYRC motifs both form part of a single folded module (the FYR domain), which adopts an α+ β fold consisting of a six-stranded antiparallel β-sheet followed by four consecutive α-helices (see <PDB:2WZO>). The FYRN region corresponds to β-strands 1-4 and their connecting loops, whereas the FYRC motif maps to β-strand 5, β-strand 6 and helices α1 to α4. Most of the conserved tyrosine and phenylalanine residues, after which these motifs are named are involved in interactions that stabilize the fold. Proteins such as MLL, in which the FYRN and FYRC regions are separated by hundreds of amino acids, are expected to contain FYR domains with a large insertion between two of the strands of the β-sheet (strands 4 and 5) [4].

The profiles we developed cover the entire FYRN and FYRC motifs.

Last update:

July 2011 / First entry.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

FYRC, PS51543; FYR domain FYRC motif profile  (MATRIX)

FYRN, PS51542; FYR domain FYRN motif profile  (MATRIX)


1AuthorsPrasad R. Zhadanov A.B. Sedkov Y. Bullrich F. Druck T. Rallapalli R. Yano T. Alder H. Croce C.M. Huebner K. Mazo A. Canaani E.
TitleStructure and expression pattern of human ALR, a novel gene with strong homology to ALL-1 involved in acute leukemia and to Drosophila trithorax.
SourceOncogene 15:549-560(1997).
PubMed ID9247308

2AuthorsBalciunas D. Ronne H.
TitleEvidence of domain swapping within the jumonji family of transcription factors.
SourceTrends Biochem. Sci. 25:274-276(2000).
PubMed ID10838566

3AuthorsDoerks T. Copley R.R. Schultz J. Ponting C.P. Bork P.
TitleSystematic identification of novel protein domain families associated with nuclear functions.
SourceGenome Res. 12:47-56(2002).
PubMed ID11779830

4AuthorsGarcia-Alai M.M. Allen M.D. Joerger A.C. Bycroft M.
TitleThe structure of the FYR domain of transforming growth factor beta regulator 1.
SourceProtein Sci. 19:1432-1438(2010).
PubMed ID20506279

5AuthorsHsieh J.J. Ernst P. Erdjument-Bromage H. Tempst P. Korsmeyer S.J.
TitleProteolytic cleavage of MLL generates a complex of N- and C-terminal fragments that confers protein stability and subnuclear localization.
SourceMol. Cell. Biol. 23:186-194(2003).
PubMed ID12482972

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