PROSITE documentation PDOC51723
Peptidase family M60 domain profile


The peptidase family M60 domain belongs to the zincin superfamily of zinc-requiring metalloproteases (clan MA, subclan MA(E)) [E1]. The peptidase family M60 domain contains the metal-binding consensus motif HExxH. The two histidine residues are ligands of the catalytic Zn(2+) and the glutamic acid residue is involved in nucleophilic attack. An additional conserved glutamic acid is found approximately 20 residues from the first histidine within the metal-binding motif. The second conserved glutamic acid potentially acts as a third proteous Zn(2+) ligand. The peptidase family M60 domain targets complex host glycoproteins, such as mucins [1,2,3,4,5].

Some proteins known to contain a peptidase family M60 domain are listed below:

  • Baculovirus viral enhancing factor (VEF), also known as Enhancin, a metalloprotease that enhances viral infection by degrading the peritrophic membrane (PM) of the insect midgut.
  • Bacteria enhancin-like metalloproteases.
  • Mammalian FAM115 family proteins.

The profile we developed covers the entire peptidase family M60 domain.

Last update:

June 2014 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

PEPTIDASE_M60, PS51723; Peptidase family M60 domain profile  (MATRIX)


1AuthorsBischoff D.S. Slavicek J.M.
TitleMolecular analysis of an enhancin gene in the Lymantria dispar nuclear polyhedrosis virus.
SourceJ. Virol. 71:8133-8140(1997).
PubMed ID9343163

2AuthorsGalloway C.S. Wang P. Winstanley D. Jones I.M.
TitleComparison of the bacterial Enhancin-like proteins from Yersinia and Bacillus spp. with a baculovirus Enhancin.
SourceJ. Invertebr. Pathol. 90:134-137(2005).
PubMed ID16081094

3AuthorsHajaij-Ellouze M. Fedhila S. Lereclus D. Nielsen-LeRoux C.
TitleThe enhancin-like metalloprotease from the Bacillus cereus group is regulated by the pleiotropic transcriptional activator PlcR but is not essential for larvicidal activity.
SourceFEMS Microbiol. Lett. 260:9-16(2006).
PubMed ID16790012

4AuthorsToprak U. Harris S. Baldwin D. Theilmann D. Gillott C. Hegedus D.D. Erlandson M.A.
TitleRole of enhancin in Mamestra configurata nucleopolyhedrovirus virulence: selective degradation of host peritrophic matrix proteins.
SourceJ. Gen. Virol. 93:744-753(2012).
PubMed ID22238230

5AuthorsNakjang S. Ndeh D.A. Wipat A. Bolam D.N. Hirt R.P.
TitleA novel extracellular metallopeptidase domain shared by animal host-associated mutualistic and pathogenic microbes.
SourcePLoS ONE 7:E30287-E30287(2012).
PubMed ID22299034


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