PROSITE documentation PDOC51839
His(Cys)3-ligated-type [4Fe-4S] domain profile


The following proteins contain a His(Cys)3-ligated-type [4Fe-4S] domain that consists of just two α helices separated by a loop region that coordinates a [4Fe-4S] cluster through an unusual H-x(3)-C-x(2)-C-x(5)-C motif that includes one His and three Cys residues (see <PDB:1FEH>) [1,2,3,4,5,6]:

  • Prokaryotic and mitochondrial NuoG/Nqo3 subunit (75 kDa) of NADH:ubiquinone oxidoreductase (complex I) (see <PDOC00554>).
  • Clostridium pasteurianum of the iron-only hydrogenase (CpI) (EC:
  • Ralstonia eutropha NAD-dependent formate dehydrogenase α subunit (FdsA).
  • Moorella thermoacetica formate dehydrogenase α subunit (FdhA).
  • Desulfovibrio fructosivorans NADP-reducing hydrogenase subunit HndD.
  • Clostridium pasteurianum
  • Ralstonia eutropha NAD-reducing [Ni-Fe] hydrogenase (H2:NAD oxidoreductase) (HoxU). Its motif for the [4Fe-4S] cluster is G-x-H-x-C-x(2)-C-x(5)-C.

The profile we developed covers the entire His(Cys)3-ligated-type [4Fe-4S] domain.

Last update:

July 2017 / First entry.


Technical section

PROSITE method (with tools and information) covered by this documentation:

4FE4S_HC3, PS51839; His(Cys)3-ligated-type [4Fe-4S] domain profile  (MATRIX)


1AuthorsOh J.-I. Bowien B.
TitleStructural analysis of the fds operon encoding the NAD+-linked formate dehydrogenase of Ralstonia eutropha.
SourceJ. Biol. Chem. 273:26349-26360(1998).
PubMed ID9756865

2AuthorsPeters J.W. Lanzilotta W.N. Lemon B.J. Seefeldt L.C.
TitleX-ray crystal structure of the Fe-only hydrogenase (CpI) from Clostridium pasteurianum to 1.8 angstrom resolution.
SourceScience 282:1853-1858(1998).
PubMed ID9836629

3AuthorsNicolet Y. Lemon B.J. Fontecilla-Camps J.C. Peters J.W.
TitleA novel FeS cluster in Fe-only hydrogenases.
SourceTrends Biochem. Sci. 25:138-143(2000).
PubMed ID10694885

4AuthorsYano T. Sklar J. Nakamaru-Ogiso E. Takahashi Y. Yagi T. Ohnishi T.
TitleCharacterization of cluster N5 as a fast-relaxing [4Fe-4S] cluster in the Nqo3 subunit of the proton-translocating NADH-ubiquinone oxidoreductase from Paracoccus denitrificans.
SourceJ. Biol. Chem. 278:15514-15522(2003).
PubMed ID12600982

5AuthorsSazanov L.A. Hinchliffe P.
TitleStructure of the hydrophilic domain of respiratory complex I from Thermus thermophilus.
SourceScience 311:1430-1436(2006).
PubMed ID16469879

6AuthorsNakamaru-Ogiso E. Matsuno-Yagi A. Yoshikawa S. Yagi T. Ohnishi T.
TitleIron-sulfur cluster N5 is coordinated by an HXXXCXXCXXXXXC motif in the NuoG subunit of Escherichia coli NADH:quinone oxidoreductase (complex I).
SourceJ. Biol. Chem. 283:25979-25987(2008).
PubMed ID18603533

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