PROSITE documentation PDOC51891CENP-V/GFA domain profile
Centrome protein (CENP)-V is a kinetochore protein that drives the progression of mitosis by coordinating chromatin condensation, positioning of sister chromatid centromeres and targeting of the chromosome passager complex (CPC), a machinery that regulates the attachment of spindle microtubules (MTs) to kinetochores. CENP-V homologues are found in all vertebrates as well as in plants and nematodes. At the C-terminal end, CENP-V possesses an evolutionally conserved domain that comprises an array of seven cysteines and shows high structural similarity to glutathione-dependent formaldehyde-activating enzyme (Gfa), an enzyme responsible for formaldehyde detoxification in prokaryotes. CENP-V could be an enzyme that scavenges the formaldehyde produced in histone demethylation reactions. The three central cysteines and the flanking four cysteines separately coordinate to zinc, forming a catalytic center and a structural fold for a tertiary structure, respectively [1,2,3].
The main topological feature of the CENP-V/GFA domain is an extremely twisted mixed eight-stranded β-sheet (see <PDB:1X6M>. Within this β-sheet, the strands β8, β9, and β4 form a sandwich with another triple stranded mixed β-sheet (β5, β2, β1). The interconnection between the sheets is mediated by four 3(10) helices and two α helices. The sandwich arrangement of the β-strands is further buttressed by a terahedral zinc coordinated by the side chains of four cysteines. It appears that this zinc atom has only a structural role. A second zinc ion stabilizes a large hairpin loop that connects β3 and β4 via residues of three cysteines [3].
The profile we developed covers the entire CENP-V/GFA domain.
Last update:April 2019 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Tadeu A.M.B. Ribeiro S. Johnston J. Goldberg I. Gerloff D. Earnshaw W.C. |
| Title | CENP-V is required for centromere organization, chromosome alignment and cytokinesis. | |
| Source | EMBO. J. 27:2510-2522(2008). | |
| PubMed ID | 18772885 | |
| DOI | 10.1038/emboj.2008.175 |
| 2 | Authors | Honda Z. Suzuki T. Honda H. |
| Title | Identification of CENP-V as a novel microtubule-associating molecule that activates Src family kinases through SH3 domain interaction. | |
| Source | Genes. Cells. 14:1383-1394(2009). | |
| PubMed ID | 19930468 | |
| DOI | 10.1111/j.1365-2443.2009.01355.x |
| 3 | Authors | Neculai A.M. Neculai D. Griesinger C. Vorholt J.A. Becker S. |
| Title | A dynamic zinc redox switch. | |
| Source | J. Biol. Chem. 280:2826-2830(2005). | |
| PubMed ID | 15548539 | |
| DOI | 10.1074/jbc.C400517200 |
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