The many bacterial transcription regulation proteins which bind DNA through a
'helix-turn-helix' motif can be classified into subfamilies on the basis of
sequence similarities. One of these subfamilies groups together the following
aarP, a transcriptional activator of the 2'-N-acetyltransferase gene in
ada, an Escherichia coli and Salmonella typhimurium bifunctional protein
that repairs alkylated guanine in DNA by transferring the alkyl group at
the O(6) position to a cysteine residue in the enzyme. The methylated
protein acts a positive regulator of its own synthesis and of the alkA,
alkB and aidB genes.
adaA, a Bacillus subtilis bifunctional protein that acts both as a
transcriptional activator of the ada operon and as a methylphosphotriester-
adiY, an Escherichia coli protein of unknown function.
aggR, the transcriptional activator of aggregative adherence fimbria I
expression in enteroaggregative Escherichia coli.
appY, a protein which acts as a transcriptional activator of acid
phosphatase and other proteins during the deceleration phase of growth and
acts as a repressor for other proteins that are synthesized in exponential
growth or in the stationary phase.
araC, the arabinose operon regulatory protein, which activates the
transcription of the araBAD genes.
cafR, the Yersinia pestis F1 operon positive regulatory protein.
celD, the Escherichia coli cel operon repressor.
cfaD, a protein which is required for the expression of the CFA/I adhesin
of enterotoxigenic Escherichia coli.
csvR, a transcriptional activator of fimbrial genes in enterotoxigenic
envY, the porin thermoregulatory protein, which is involved in the control
of the temperature-dependent expression of several Escherichia coli
envelope proteins such as ompF, ompC, and lamB.
exsA, an activator of exoenzyme S synthesis in Pseudomonas aeruginosa.
fapR, the positive activator for the expression of the 987P operon coding
for the fimbrial protein in enterotoxigenic Escherichia coli.
hrpB, a positive regulator of pathogenicity genes in Burkholderia
invF, the Salmonella typhimurium invasion operon regulator.
marA, which may be a transcriptional activator of genes involved in the
multiple antibiotic resistance (mar) phenotype.
melR, the melibiose operon regulatory protein, which activates the
transcription of the melAB genes.
mixE, a Shigella flexneri protein necessary for secretion of ipa invasins.
mmsR, the transcriptional activator for the mmsAB operon in Pseudomonas
msmR, the multiple sugar metabolism operon transcriptional activator in
pchR, a Pseudomonas aeruginosa activator for pyochelin and ferripyochelin
perA, a transcriptional activator of the eaeA gene for intimin in
enteropathogenic Escherichia coli.
pocR, a Salmonella typhimurium regulator of the cobalamin biosynthesis
pqrA, from Proteus vulgaris.
rafR, the regulator of the raffinose operon in Pediococcus pentosaceus.
ramA, from Klebsiella pneumoniae.
rhaR, the Escherichia coli and Salmonella typhimurium L-rhamnose operon
rhaS, an Escherichia coli and Salmonella typhimurium positive activator of
genes required for rhamnose utilization.
rns, a protein which is required for the expression of the cs1 and cs2
adhesins of enterotoxigenic Escherichia coli.
rob, a protein which binds to the right arm of the replication origin oriC
of the Escherichia coli chromosome.
soxS, a protein that, with the soxR protein, controls a superoxide response
regulon in Escherichia coli.
tetD, a protein from transposon TN10.
tcpN or toxT, the Vibrio cholerae transcriptional activator of the tcp
operon involved in pilus biosynthesis and transport.
thcR, a probable regulator of the thc operon for the degradation of the
thiocarbamate herbicide EPTC in Rhodococcus sp. strain NI86/21.
ureR, the transcriptional activator of the plasmid-encoded urease operon in
virF and lcrF, the Yersinia virulence regulon transcriptional activator.
virF, the Shigella transcriptional factor of invasion related antigens
xylR, the Escherichia coli xylose operon regulator.
xylS, the transcriptional activator of the Pseudomonas putida TOL plasmid
(pWWO, pWW53 and pDK1) meta operon (xylDLEGF genes).
yfeG, an Escherichia coli hypothetical protein.
yhiW, an Escherichia coli hypothetical protein.
yhiX, an Escherichia coli hypothetical protein.
yidL, an Escherichia coli hypothetical protein.
yijO, an Escherichia coli hypothetical protein.
yuxC, a Bacillus subtilis hypothetical protein.
yzbC, a Bacillus subtilis hypothetical protein.
Except for celD, all of these proteins seem to be positive transcriptional
factors. Their size range from 107 (soxS) to 529 (yzbC) residues.
The helix-turn-helix motif is located in the third quarter of most of the
sequences; the N-terminal and central regions of these proteins are presumed
to interact with effector molecules and may be involved in dimerization. The
minimal DNA binding domain, which spans roughly 100 residues and comprises the
HTH motif contains another region with similarity to classical HTH domain.
However, it contains an insertion of one residue in the turn-region.
A signature pattern was derived from the region that follows the first HTH
domain and that includes the totality of the putative second HTH domain. A
more sensitive detection of members of the araC family is available through
the use of a profile which spans the minimal DNA-binding region of 100
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and
distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives
(CC BY-NC-ND 4.0) License, see prosite_license.html.