|PROSITE documentation PDOC50940 [for PROSITE entry PS50940]|
The species distribution of the chitin-binding type-2 domain is complementary to the chitin-binding type-1 domain (see <PDOC00025>). The type-2 is mainly found in animal and baculovirus proteins while the type-1 is found in plant and fungi. Type-2 is characterized by having a 6-cysteine motif: C-x(13,20)-C-x(5,6)-C-x(9,19)-C-x(10,14)-C-x(4,14)-C and several aromatic residues .
The structure of the chitin-binding domain of tachycitin has been solved (see <PDB:1DQC>) . It revealed that tachycitin shares a remarkable structural similarity with the chitin-binding type-1 domain of hevein. The overall structure forms a distorted β-sandwich constructed by three-stranded and two-stranded β-sheets connected through a bending loop (Cys-40-Leu-44). The precise chitin binding site (Cys-40 to Gly-60) is perfectly superposable with the chitin binding site of hevein. Furthermore a disulfide bridge connecting Cys-40 and Cys-53 is also present at the same location in the hevein structure .
Some protein known to contain a chitin-binding type-2 domain are listed below:
The profile we developed covers the whole domain.Last update:
July 2016 / Profile revised.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Gaines P.J. Walmsley S.J. Wisnewski N.|
|Title||Cloning and characterization of five cDNAs encoding peritrophin-A domains from the cat flea, Ctenocephalides felis.|
|Source||Insect Biochem. Mol. Biol. 33:1061-1073(2003).|
|2||Authors||Suetake T. Tsuda S. Kawabata S. Miura K. Iwanaga S. Hikichi K. Nitta K. Kawano K.|
|Title||Chitin-binding proteins in invertebrates and plants comprise a common chitin-binding structural motif.|
|Source||J. Biol. Chem. 275:17929-17932(2000).|
|3||Authors||Tjoelker L.W. Gosting L. Frey S. Hunter C.L. Trong H.L. Steiner B. Brammer H. Gray P.W.|
|Title||Structural and functional definition of the human chitinase chitin-binding domain.|
|Source||J. Biol. Chem. 275:514-520(2000).|