RNA interference (RNAi) is an ancient gene-silencing process that plays a
fundamental role in diverse eukaryotic functions including viral defense,
chromatin remodeling, genome rearrangement, developmental timing, brain
morphogenesis, and stem cell maintenance. All RNAi pathways require the
multidomain ribonuclease Dicer, which initiates RNAi by cleaving double-stranded RNA (dsRNA) substrates into small fragments ~25 nuleotides in length.
A typical eukaryotic Dicer consists of a helicase domain (see <PDOC51192>), a
domain of unknown function, and a PAZ domain (see <PDOC50821>) at the amino
(N)-terminus as well as two ribonuclease III domains (see <PDOC00448>) and a
dsRNA-binding domain (dsRBD) (see <PDOC50137>) at the carboxy (C)-terminus.
The domain of unknown function of ~100 amino acids is predicted to adopt the
canonical α-β-β-β-α-fold found in all dsRBDs [1,2,3,4].
The profile we developed covers the entire Dicer dsRNA-binding fold domain.
August 2007 / First entry.
PROSITE method (with tools and information) covered by this documentation:
MacRae I.J. Zhou K. Li F. Repic A. Brooks A.N. Cande W.Z. Adams P.D. Doudna J.A.
Structural basis for double-stranded RNA processing by Dicer.
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