PROSITE documentation PDOC51521 [for PROSITE entry PS51521]Herpesvirus tegument ubiquitin-specific protease (htUSP) domain profile
All herpesviruses [E1] contain a Ubiquitin (Ub)-specific cysteine protease (USP) domain embedded within their large tegument protein. The herpesvirus tegument ubiquitin (Ub)-specific protease (htUSP) domain of ~200 amino acids adopts an α-β-α sandwich fold that features a central catalytic cleft, ideally suited to accommodate the C-terminal stretch of Ub (see <PDB:2J7Q>). The catalytic triad Cys-His-Asp is strictly conserved, along with a putative oxyanion hole-forming Gln residue. The htUSP domain is a member of peptidase family C76 of clan CA [1,2,3,4,5,E2].
The profile we developed covers the entire htUSP domain.
Last update:January 2011 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Schlieker C. Korbel G.A. Kattenhorn L.M. Ploegh H.L. |
| Title | A deubiquitinating activity is conserved in the large tegument protein of the herpesviridae. | |
| Source | J. Virol. 79:15582-15585(2005). | |
| PubMed ID | 16306630 | |
| DOI | 10.1128/JVI.79.24.15582-15585.2005 |
| 2 | Authors | Schlieker C. Weihofen W.A. Frijns E. Kattenhorn L.M. Gaudet R. Ploegh H.L. |
| Title | Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. | |
| Source | Mol. Cell 25:677-687(2007). | |
| PubMed ID | 17349955 | |
| DOI | 10.1016/j.molcel.2007.01.033 |
| 3 | Authors | Gredmark S. Schlieker C. Quesada V. Spooner E. Ploegh H.L. |
| Title | A functional ubiquitin-specific protease embedded in the large tegument protein (ORF64) of murine gammaherpesvirus 68 is active during the course of infection. | |
| Source | J. Virol. 81:10300-10309(2007). | |
| PubMed ID | 17634221 | |
| DOI | 10.1128/JVI.01149-07 |
| 4 | Authors | Gonzalez C.M. Wang L. Damania B. |
| Title | Kaposi's sarcoma-associated herpesvirus encodes a viral deubiquitinase. | |
| Source | J. Virol. 83:10224-10233(2009). | |
| PubMed ID | 19640989 | |
| DOI | 10.1128/JVI.00589-09 |
| 5 | Authors | Kim E.T. Oh S.E. Lee Y.-O. Gibson W. Ahn J.-H. |
| Title | Cleavage specificity of the UL48 deubiquitinating protease activity of human cytomegalovirus and the growth of an active-site mutant virus in cultured cells. | |
| Source | J. Virol. 83:12046-12056(2009). | |
| PubMed ID | 19759126 | |
| DOI | 10.1128/JVI.00411-09 |
| E1 | Title | https://viralzone.expasy.org/176?outline=all_by_species |
| E2 | Title | https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=c76 |
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