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PROSITE documentation PDOC51959 [for PROSITE entry PS51959]

Eukaryotic uridylate-specific endoribonuclease (EndoU) domain profile





Description

EndoU is a family of metal-dependent endoribonucleases that is broadly conserved among eukaryotes [1,2]. EndoU family members have RNA-binding and endoribonuclease activities and appear to be involved in many aspects of biology, including small nucleolar RNA biogenesis, endoplasmic reticulum (ER) network formation, immune response, and neurodegeneration:

  • Xenopus laevis endoribonuclease XendoU is responsible for processing the intron encoded U16 and U86 small nucleolar RNAs (snoRNAs) [3,4].
  • Human EndoU, also known as PP11 (placental protein 11), has an endoribonuclease activity with placental tissue specificity [5,6].
  • Drosophila melanogaster CG2145 and DendoU endoribonucleases [7].
  • Caenorhabditis elegans endu-2 regulates nucleotide metabolism and germ cell proliferation in response to nucleotide imbalance and other genotoxic stress [8].

EndoU family members share a common domain, that carries out a conserved RNA processing function. The EndoU domain cleaves RNA at uridylates and release 2',3'-cyclic phosphodiester ends.

The EndoU domain is an α+β domain, that contains nine α-helices and three antiparallel β-sheets; the latter are clustered on one side of the domain, whereas the α-helices are largely on the other side (see <PDB:2C1W>) [3]. It contains a conserved trio of catalytic residues, two histidines and a lysine.

The profile we developed covers the entire EndoU domain.

Last update:

January 2021 / First entry.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

ENDOU, PS51959; Eukaryotic uridylate-specific endoribonuclease (EndoU) domain profile  (MATRIX)


References

1AuthorsMushegian A. Sorokina I. Eroshkin A. Dlakic M.
TitleAn ancient evolutionary connection between Ribonuclease A and EndoU families.
SourceRNA 26:803-813(2020).
PubMed ID32284351
DOI10.1261/rna.074385.119

2AuthorsRagno R. Gioia U. Laneve P. Bozzoni I. Mai A. Caffarelli E.
TitleIdentification of small-molecule inhibitors of the XendoU endoribonucleases family.
SourceChemMedChem 6:1797-1805(2011).
PubMed ID21805647
DOI10.1002/cmdc.201100281

3AuthorsRenzi F. Caffarelli E. Laneve P. Bozzoni I. Brunori M. Vallone B.
TitleThe structure of the endoribonuclease XendoU: From small nucleolar RNA processing to severe acute respiratory syndrome coronavirus replication.
SourceProc. Natl. Acad. Sci. U. S. A. 103:12365-12370(2006).
PubMed ID16895992
DOI10.1073/pnas.0602426103

4AuthorsSchwarz D.S. Blower M.D.
TitleThe calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation.
SourceJ. Cell. Biol. 207:41-57(2014).
PubMed ID25287301
DOI10.1083/jcb.201406037

5AuthorsLaneve P. Gioia U. Ragno R. Altieri F. Di Franco C. Santini T. Arceci M. Bozzoni I. Caffarelli E.
TitleThe tumor marker human placental protein 11 is an endoribonuclease.
SourceJ. Biol. Chem. 283:34712-34719(2008).
PubMed ID18936097
DOI10.1074/jbc.M805759200

6AuthorsPoe J.C. Kountikov E.I. Lykken J.M. Natarajan A. Marchuk D.A. Tedder T.F.
TitleEndoU is a novel regulator of AICD during peripheral B cell selection.
SourceJ. Exp. Med. 211:57-69(2014).
PubMed ID24344237
DOI10.1084/jem.20130648

7AuthorsLaneve P. Piacentini L. Casale A.M. Capauto D. Gioia U. Cappucci U. Di Carlo V. Bozzoni I. Di Micco P. Morea V. Di Franco C.A. Caffarelli E.
TitleDrosophila CG3303 is an essential endoribonuclease linked to TDP-43-mediated neurodegeneration.
SourceSci. Rep. 7:41559-41559(2017).
PubMed ID28139767
DOI10.1038/srep41559

8AuthorsJia F. Chi C. Han M.
TitleRegulation of Nucleotide Metabolism and Germline Proliferation in Response to Nucleotide Imbalance and Genotoxic Stresses by EndoU Nuclease.
SourceCell. Rep. 30:1848-1861.e5(2020).
PubMed ID32049015
DOI10.1016/j.celrep.2020.01.050



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