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ProRule PRU00098


General rule information [?]

Accession PRU00098
Dates 12-DEC-2003 (Created)
23-FEB-2024 (Last updated, Version 11)
Data class Domain;
Predictors PROSITE; PS50221; GAIN_B
Name GAIN-B domain
Function The ~320-residue GAIN domain is an ancient domain that exists in primitive ancestor organisms, and is conserved in all adhesion-G-protein- coupled receptors (aGPCRs), all polycystic kidney disease 1 (PKD1)-like proteins and in other membrane-associated proteins like the sea urchin receptor for egg jelly protein (REJ). Functionally, the GAIN domain is both necessary and sufficient for autoproteolysis, suggesting an autoproteolytic mechanism whereby the overall GAIN domain fine-tunes the chemical environment.
Scope(s) Eukaryota
Metazoa
Example(s) Q8IZF7 (AGRF2_HUMAN);

Propagated annotation [?]

Comments [?]

case <Feature:PS50221:145-147=H-[LM]-[TSC]>
SUBUNITHeterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C- terminal fragment predominantly remain associated and non-covalently linked.
PTMAutoproteolytically processed at the GPS region of the GAIN-B domain; this cleavage modulates receptor activity.
end case

Keywords [?]

Glycoprotein
Membrane
Transmembrane
case <FTTag:disulf>
Disulfide bond
end case

Gene Ontology [?]

GO:0016020; Cellular component:membrane

Features [?]

From: PS50221
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="GAIN-B #"
REGION 112 to /note="GPS #"
REGION 147 to /note="Stachel #"
case <Feature:PS50221:145-147=H-[LM]-[TSC]>
SITE 146 147 /note="Cleavage; by autolysis"
end case
DISULFID 112 141 disulf C-x*-C
DISULFID 129 143 disulf C-x*-C

Additional information [?]

Size range 47-59 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

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UniProtKB rule member sequences [?]