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annotation rule: PRU00764

General rule information [?]

Accession PRU00764
Dates 2-MAR-2009 (Created)
14-MAY-2022 (Last updated, Version 10)
Data class Domain
Predictors PROSITE; PS51435; AP_NUCLEASE_F1_4
Name AP endonucleases family 1
Function Exonuclease III and AP endonuclease 1 related enzymes play a role in DNA repair. These nucleases cleave phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues.

Propagated annotation [?]


Description [?]

case <OC:Eukaryota>
AltName: Full=DNA-(apurinic or apyrimidinic site) endonuclease; EC 3.1.-.-;
else case <OC:Bacteria>
AltName: Full=Exodeoxyribonuclease III; Short=Exonuclease III; Short=EXO III; EC 3.1.11.2;
end case


Comments [?]

case <OC:Eukaryota>
Subcellular location Nucleus.
else case <OC:Bacteria>
Catalytic activity Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.; EC=3.1.11.2;
Subcellular location Cytoplasm.
end case

Similarity Belongs to the DNA repair enzymes AP/exoA family.

Cross-references [?]

case <Feature:PS51435:40=E>
PROSITE PS00726; AP_NUCLEASE_F1_1; 1;
end case

PROSITE PS00727; AP_NUCLEASE_F1_2; 1;
PS00728; AP_NUCLEASE_F1_3; 1;

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0004519; Molecular function: endonuclease activity.
GO:0006281; Biological process: DNA repair.
case <OC:Eukaryota>
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0005634; Cellular component: nucleus.
else case <OC:Bacteria>
GO:0000287; Molecular function: magnesium ion binding.
GO:0008853; Molecular function: exodeoxyribonuclease III activity.
GO:0005737; Cellular component: cytoplasm.
end case


Keywords [?]

DNA damage
DNA repair
Hydrolase
Metal-binding
Nuclease
case <OC:Eukaryota>
Endonuclease
Nucleus
else case <OC:Bacteria>
Cytoplasm
Exonuclease
Magnesium
end case


Features [?]

From: PS51435
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     258     258       Proton acceptor     H  
BINDING     7     7       /ligand="Mg(2+)" /ligand_id="18420     N  
BINDING     40     40       /ligand="Mg(2+)" /ligand_id="18420     E  
BINDING     158     158       /ligand="Mg(2+)" /ligand_id="18420     D  
BINDING     160     160       /ligand="Mg(2+)" /ligand_id="18420     N  
BINDING     257     257       /ligand="Mg(2+)" /ligand_id="18420     D  
BINDING     258     258       /ligand="Mg(2+)" /ligand_id="18420     H  

Additional information [?]

Size range 230-370 amino acids
Related rules None
Repeats 1
Topology Undefined
Examples P28352 (APEX1_MOUSE); P44318 (EX3_HAEIN): [Recover all]
Scope
Eukaryota
Bacteria
Comments None

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.


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UniProtKB rule member sequences [?]