PRU00764

General rule information [?]

Accession	PRU00764
Dates	2-MAR-2009 (Created) 13-FEB-2023 (Last updated, Version 12)

Data class

Domain

Predictors

PROSITE; PS51435; AP_NUCLEASE_F1_4

Name	AP endonucleases family 1

Function

Exonuclease III and AP endonuclease 1 related enzymes play a role in DNA repair. These nucleases cleave phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues.

Propagated annotation [?]

Description [?]

case <OC:Eukaryota>

AltName: Full=DNA-(apurinic or apyrimidinic site) endonuclease; EC 3.1.-.-;

else case <OC:Bacteria>

AltName: Full=Exodeoxyribonuclease III; Short=Exonuclease III; Short=EXO III; EC 3.1.11.2;

end case

Comments [?]

case <OC:Eukaryota>

Subcellular location

Nucleus.

else case <OC:Bacteria>

Catalytic activity	Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.; EC=3.1.11.2;
Subcellular location	Cytoplasm.

end case

Similarity

Belongs to the DNA repair enzymes AP/exoA family.

Cross-references [?]

case <Feature:PS51435:40=E>

PROSITE

PS00726; AP_NUCLEASE_F1_1; 1;

end case

PROSITE	PS00727; AP_NUCLEASE_F1_2; 1;
	PS00728; AP_NUCLEASE_F1_3; 1;

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0004519; Molecular function: endonuclease activity.
GO:0006281; Biological process: DNA repair.

case <OC:Eukaryota>

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0005634; Cellular component: nucleus.

else case <OC:Bacteria>

GO:0000287; Molecular function: magnesium ion binding.
GO:0008311; Molecular function: double-stranded DNA 3'-5' DNA exonuclease activity.
GO:0005737; Cellular component: cytoplasm.

end case

Keywords [?]

DNA damage
DNA repair
Hydrolase
Metal-binding
Nuclease

case <OC:Eukaryota>

Endonuclease
Nucleus

else case <OC:Bacteria>

Cytoplasm
Exonuclease
Magnesium

end case

Features [?]

From: PS51435

Key From To Description Tag Condition FTGroup

ACT_SITE 258 258 Proton acceptor H

BINDING 7 7 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 N

BINDING 40 40 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 E

BINDING 158 158 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 160 160 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 N

BINDING 257 257 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 258 258 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 H

Additional information [?]

Size range	230-370 amino acids
Related rules	None
Repeats	1
Topology	Undefined
Examples	P28352 (APEX1_MOUSE); P44318 (EX3_HAEIN): [Recover all]
Scope	Eukaryota Bacteria
Comments	None

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

View rule in raw text format (no links)

UniProtKB rule member sequences [?]

UniProtKB/Swiss-Prot sets

Archaea [1]

Bacteria [7]

Eukaryota [21]

All [ 29 ]
Retrieve set of proteins with 3D structure for this domain

Archaea	[1]
Bacteria	[7]
Eukaryota	[21]
All	[ 29 ]