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annotation rule: PRU00764

General rule information [?]

Accession PRU00764
Dates 2-MAR-2009 (Created)
27-OCT-2018 (Last updated, Version 6)
Data class Domain
Predictors PROSITE; PS51435; AP_NUCLEASE_F1_4
Name AP endonucleases family 1
Function Exonuclease III and AP endonuclease 1 related enzymes play a role in DNA repair. These nucleases cleave phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues.

Propagated annotation [?]


Description [?]

case <OC:Eukaryota>
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase; EC=4.2.99.18;
else case <OC:Bacteria>
AltName: Full=Exodeoxyribonuclease III; Short=Exonuclease III; Short=EXO III; EC=3.1.11.2;
end case


Comments [?]

case <OC:Eukaryota>
Catalytic activity Reaction=The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'- terminal unsaturated sugar and a product with a terminal 5'- phosphate.; EC=4.2.99.18;.
Subcellular location Nucleus.
else case <OC:Bacteria>
Catalytic activity Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.; EC=3.1.11.2;.
Subcellular location Cytoplasm.
end case

Similarity Belongs to the DNA repair enzymes AP/exoA family.

Cross-references [?]

case <Feature:PS51435:40=E>
PROSITE PS00726; AP_NUCLEASE_F1_1; 1;
end case

PROSITE PS00727; AP_NUCLEASE_F1_2; 1;
PS00728; AP_NUCLEASE_F1_3; 1;

Gene Ontology [?]

GO:0003677; Molecular function: DNA binding.
GO:0004519; Molecular function: endonuclease activity.
GO:0006281; Biological process: DNA repair.
case <OC:Eukaryota>
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) endonuclease activity.
GO:0005634; Cellular component: nucleus.
else case <OC:Bacteria>
GO:0000287; Molecular function: magnesium ion binding.
GO:0008853; Molecular function: exodeoxyribonuclease III activity.
GO:0005737; Cellular component: cytoplasm.
end case


Keywords [?]

case <OC:Eukaryota>
else case <OC:Bacteria>
end case


Features [?]

From: PS51435
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     258     258       Proton acceptor     H  
METAL     7     7       Magnesium or manganese     N  
METAL     40     40       Magnesium or manganese     E  
METAL     158     158       Magnesium or manganese     D  
METAL     160     160       Magnesium or manganese     N  
METAL     257     257       Magnesium or manganese     D  
METAL     258     258       Magnesium or manganese     H  

Additional information [?]

Size range 230-370 amino acids
Related rules None
Repeats 1
Topology Undefined
Examples P28352 (APEX1_MOUSE); P44318 (EX3_HAEIN): [Recover all]
Scope
Eukaryota
Bacteria
Comments None

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.



UniProtKB rule member sequences [?]