ProRule PRU00860

General rule information [?]

Accession PRU00860
Dates 9-JUN-2011 (Created)
21-NOV-2019 (Last updated, Version 6)
Data class Domain;
Name Flavivirus NS3 protease (NS3pro) domain
Function The NS3pro has a classical serine protease catalytic triad (His, Asp, and Ser). The enzymatic activity of NS3pro is enhanced by interacting with the central 40 amino acid of NS2B which acts as an essential cofactor.
Scope(s) Viruses
Example(s) P07564 (POLG_DEN2J);

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
FUNCTIONSerine protease NS3: Displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B- NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction.
SUBCELLULAR LOCATIONSerine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Remains non-covalently associated to serine protease subunit NS2B.
end case

Keywords [?]

Gene Ontology [?]

case <FTGroup:1>
GO:0016787; Molecular function:hydrolase activity
GO:0008233; Molecular function:peptidase activity
GO:0008236; Molecular function:serine-type peptidase activity

Features [?]

From: PS51528
Key From To Description Tag Condition FTGroup
CHAIN ? ? /note="Serine protease NS3"
DOMAIN from to /note="Peptidase S7 #"
ACT_SITE 53 53 /note="Charge relay system; for serine protease NS3 activity" H 1
ACT_SITE 77 77 /note="Charge relay system; for serine protease NS3 activity" D 1
ACT_SITE 137 137 /note="Charge relay system; for serine protease NS3 activity" S 1

Additional information [?]

Size range 168-191 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined


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UniProtKB rule member sequences [?]