ProRule PRU00895

General rule information [?]

Accession PRU00895
Dates 15-MAR-2012 (Created)
11-JAN-2024 (Last updated, Version 28)
Data class Domain;
Predictors PROSITE; PS51562; RNA_CAP0_MT
Name mRNA (guanine-N(7))-methyltransferase (EC
Function mRNA (guanine-N(7))-methyltransferase (EC is an enzyme that transfers a methyl group from S-adenosyl-L-methionine (SAM) to G(5')pppR-RNA to form m(7)G(5')pppR-RNA and S-adenosyl-L-homocysteine.
Scope(s) Eukaryota
Example(s) Q8SR66 (MCES_ENCCU); P04298 (MCEL_VACCW);

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name + RecName: EC=;

Comments [?]

CATALYTIC ACTIVITY Reaction=a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S- adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'- triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA- COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461, ChEBI:CHEBI:167617; EC=;
case <OC:Eukaryota>
SIMILARITYBelongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.
else case <OC:Viruses>
SIMILARITYIn the C-terminal section; belongs to the class I-like SAM- binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.
end case

Keywords [?]

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding
GO:0004482; Molecular function:mRNA 5'-cap (guanine-N7-)-methyltransferase activity
GO:0006370; Biological process:7-methylguanosine mRNA capping
GO:0032259; Biological process:methylation
GO:0046500; Biological process:S-adenosylmethionine metabolic process

Features [?]

From: PS51562
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="mRNA cap 0 methyltransferase #"
BINDING 10 11 /ligand="mRNA"
/ligand_part="mRNA cap"
case <OC:Eukaryota>
BINDING 91 92 /ligand="S-adenosyl-L-methionine"
end case
BINDING 117 119 /ligand="S-adenosyl-L-methionine"
BINDING 14 14 /ligand="S-adenosyl-L-methionine"
BINDING 35 35 /ligand="S-adenosyl-L-methionine"
BINDING 57 57 /ligand="S-adenosyl-L-methionine"
SITE 38 38 /note="mRNA cap binding" K
SITE 44 44 /note="mRNA cap binding" K
SITE 69 69 /note="mRNA cap binding" R
SITE 121 121 /note="mRNA cap binding" H
SITE 209 209 /note="mRNA cap binding" E
SITE 278 278 /note="mRNA cap binding" Y

Additional information [?]

Size range 220-365 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined


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UniProtKB rule member sequences [?]