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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01211


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01211
General rule information [?]

Accession PRU01211
Dates 05-JUN-2018 (Created)
19-NOV-2022 (Last updated, Version 5)
Data class Domain;
Predictors PROSITE; PS51864; ASTACIN
Name Astacin-like domain
Function Astacin or M12A family zinc metalloproteases are found in bacteria and animals. They belong to the metzincin protease family and are synthesized as inactive precursors. The zinc atom in these enzymes acts as an electrophilic center to enhance the reactivity of a water molecule prior to nucleophilic attack on the carbonyl carbon of the scissile peptide bond.
Scope(s) Eukaryota
Bacteria
Example(s) P07584;

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit
end case

Keywords [?]

case <FTTag:catalytic> and <FTGroup:1>
Hydrolase
Protease
Metalloprotease
end case
case <FTGroup:1>
Zinc
Metal-binding
end case
case <FTTag:disulf>
Disulfide bond
end case

Gene Ontology [?]

GO:0004222; Molecular function:metalloendopeptidase activity
end case
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case

Cross-references [?]

PROSITE PS00142; ZINC_PROTEASE; 1;

Features [?]

From: PS51864
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Peptidase M12A #"
ACT_SITE 95 95 catalytic E
BINDING 94 94 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 H 1
BINDING 98 98 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 H 1
BINDING 104 104 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_note="catalytic"		 H 1
DISULFID 5 8 disulf C-x*-C
DISULFID 43 198 disulf C-x*-C
DISULFID 64 86 disulf C-x*-C
DISULFID 66 67 disulf C-x*-C

Additional information [?]

Size range 185-215 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]