ProRule PRU01293
General rule information
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Accession | PRU01293 |
Dates | 1-DEC-2020 (Created)
19-NOV-2022 (Last updated, Version 5) |
Data class | Domain; |
Predictors |
PROSITE; PS51948; COV_NSP12_RDRP |
Name | Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain |
Function | The Nsp12 RNA-dependent RNA polymerase, that includes an RdRp catalytic domain conserved in all RNA viruses, possesses some minimal activity on its own, but the addition of the Nsp7 and Nsp8 cofactors greatly stimulates polymerase activity. |
Scope(s) |
Viruses Orthocoronavirinae |
Example(s) | P0C6Y1 (R1AB_IBVB); |
Propagated annotation
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Identifier, protein and gene names
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case <FTGroup:1> | |
Protein name | + AltName: Full=RNA-directed RNA polymerase; EC=2.7.7.48; |
Comments
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CATALYTIC ACTIVITY | [RNA-directed RNA polymerase]: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; |
end case | |
SIMILARITY | Belongs to the coronaviruses polyprotein 1ab family. |
Keywords
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Nucleotidyltransferase | |
RNA-directed RNA polymerase | |
Transferase | |
end case | |
RNA-binding | |
Viral RNA replication | |
Nucleotide-binding | |
case <FTGroup:2> | |
Zinc | |
Metal-binding | |
end case |
Gene Ontology
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GO:0000166; Molecular function:nucleotide binding | |
GO:0003723; Molecular function:RNA binding | |
GO:0039694; Biological process:viral RNA genome replication | |
case <FTGroup:1> | |
GO:0016740; Molecular function:transferase activity | |
GO:0016779; Molecular function:nucleotidyltransferase activity | |
GO:0003968; Molecular function:RNA-dependent RNA polymerase activity |
Features
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From: PS51948 | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
DOMAIN | from | to | /note="Nsp12 RNA-dependent RNA polymerase #" | |||||||||
REGION | 3 | 217 | /note="RdRp Fingers N-ter #" | |||||||||
REGION | 218 | 256 | /note="RdRp Palm N-ter #" | |||||||||
REGION | 257 | 315 | /note="RdRp Fingers C-ter #" | |||||||||
REGION | 316 | 451 | /note="RdRp Palm C-ter #" | |||||||||
REGION | 452 | 568 | /note="RdRp Thumb #" | |||||||||
ACT_SITE | 395 | 395 | S | 1 | ||||||||
ACT_SITE | 396 | 396 | D | 1 | ||||||||
ACT_SITE | 397 | 397 | D | 1 | ||||||||
BINDING | 123 | 123 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1" |
[CH] | 2 | |||||||
BINDING | 278 | 278 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1" |
[HC] | 2 | |||||||
BINDING | 281 | 281 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1" |
[CH] | 2 | |||||||
BINDING | 282 | 282 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1" |
[CH] | 2 |
Additional information
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Size range | 560-570 amino acids |
Related rules |
None |
Fusion | None |
Repeats | 1 |
Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Viruses [32] All [ 32 ]
- Retrieve set of proteins with 3D structure for this domain