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annotation rule: PRU01293

General rule information [?]

Accession PRU01293
Dates 1-DEC-2020 (Created)
19-NOV-2022 (Last updated, Version 5)
Data class Domain
Predictors PROSITE; PS51948; COV_NSP12_RDRP
Name Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain
Function The Nsp12 RNA-dependent RNA polymerase, that includes an RdRp catalytic domain conserved in all RNA viruses, possesses some minimal activity on its own, but the addition of the Nsp7 and Nsp8 cofactors greatly stimulates polymerase activity.

Propagated annotation [?]


Description [?]

case <FTGroup:1>
+ AltName: Full=RNA-directed RNA polymerase; EC 2.7.7.48;

Comments [?]

Catalytic activity [RNA-directed RNA polymerase]: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48;
end case

Similarity Belongs to the coronaviruses polyprotein 1ab family.

Gene Ontology [?]

GO:0000166; Molecular function: nucleotide binding.
GO:0003723; Molecular function: RNA binding.
GO:0039694; Biological process: viral RNA genome replication.
case <FTGroup:1>
GO:0016740; Molecular function: transferase activity.
GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0003968; Molecular function: RNA-dependent RNA polymerase activity.

Keywords [?]

Nucleotidyltransferase
RNA-directed RNA polymerase
Transferase
end case

RNA-binding
Viral RNA replication
Nucleotide-binding
case <FTGroup:2>
Zinc
Metal-binding
end case


Features [?]

From: PS51948
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     from     to       Nsp12 RNA-dependent RNA polymerase #        
REGION     3     217       RdRp Fingers N-ter #        
REGION     218     256       RdRp Palm N-ter #        
REGION     257     315       RdRp Fingers C-ter #        
REGION     316     451       RdRp Palm C-ter #        
REGION     452     568       RdRp Thumb #        
ACT_SITE     395     395             S   1
ACT_SITE     396     396             D   1
ACT_SITE     397     397             D   1
BINDING     123     123       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1     [CH]   2
BINDING     278     278       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1     [HC]   2
BINDING     281     281       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1     [CH]   2
BINDING     282     282       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1     [CH]   2

Additional information [?]

Size range 560-570 amino acids
Related rules None
Repeats 1
Topology Undefined
Example P0C6Y1 (R1AB_IBVB)
Scope
Viruses; Orthocoronavirinae
Comments None

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.


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UniProtKB rule member sequences [?]