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ProRule PRU01293


General rule information [?]

PURL https://purl.expasy.org/prosite/rule/PRU01293
Accession PRU01293
Dates 1-DEC-2020 (Created)
20-MAR-2025 (Last updated, Version 7)
Data class Domain;
Predictors PROSITE; PS51948; COV_NSP12_RDRP
Name Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain
Function The Nsp12 RNA-dependent RNA polymerase, that includes an RdRp catalytic domain conserved in all RNA viruses, possesses some minimal activity on its own, but the addition of the Nsp7 and Nsp8 cofactors greatly stimulates polymerase activity.
Scope(s) Viruses
Orthocoronavirinae
Example(s) P0C6Y1 (R1AB_IBVB);

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTGroup:1>
Protein name + AltName: Full=RNA-directed RNA polymerase;
                 EC=2.7.7.48;

Comments [?]

CATALYTIC ACTIVITY[RNA-directed RNA polymerase]: Reaction=RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate; Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48;
end case
SIMILARITYBelongs to the coronaviruses polyprotein 1ab family.

Keywords [?]

Nucleotidyltransferase
RNA-directed RNA polymerase
Transferase
end case
RNA-binding
Viral RNA replication
Nucleotide-binding
case <FTGroup:2>
Zinc
Metal-binding
end case

Gene Ontology [?]

GO:0000166; Molecular function:nucleotide binding
GO:0003723; Molecular function:RNA binding
GO:0039694; Biological process:viral RNA genome replication
case <FTGroup:1>
GO:0016740; Molecular function:transferase activity
GO:0016779; Molecular function:nucleotidyltransferase activity
GO:0003968; Molecular function:RNA-directed RNA polymerase activity

Features [?]

From: PS51948
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Nsp12 RNA-dependent RNA polymerase #"
REGION 3 217 /note="RdRp Fingers N-ter #"
REGION 218 256 /note="RdRp Palm N-ter #"
REGION 257 315 /note="RdRp Fingers C-ter #"
REGION 316 451 /note="RdRp Palm C-ter #"
REGION 452 568 /note="RdRp Thumb #"
ACT_SITE 395 395 S 1
ACT_SITE 396 396 D 1
ACT_SITE 397 397 D 1
BINDING 123 123 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 2
BINDING 278 278 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [HC] 2
BINDING 281 281 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 2
BINDING 282 282 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 2

Additional information [?]

Size range 560-570 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.



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UniProtKB rule member sequences [?]