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General rule information [?]

Accession PRU01364
Dates 17-MAR-2023 (Created)
17-MAR-2023 (Last updated, Version 1)
Data class Domain
Predictors PROSITE; PS52020; CRESS_DNA_REP
Name CRESS-DNA virus replication initiator protein (Rep) endonuclease domain
Function The Rep endonuclease domain is characterized by rolling circle replication (RCR) motifs I through III, which are important for RCR initiation and termination. Motif I, UUTU (U denotes hydrophobic residues), is thought to be involved in the recognition of the origin of replication. Motif II, HU(H/Q), is involved in the coordination of divalent metal ions, Mg(2+) or Mn(2+), which are essential for endonuclease activity at the origin of replication. Motif III (YxxK, where x is any amino acid) is involved in dsDNA cleavage and subsequent covalent attachment of the Rep through the catalytic tyrosine residue to the 5' end of the cleaved product.

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
Cofactor Mg(2+)
Note: Divalent metal cations, possibly Mg(2+) or Mn(2+)
end case

Subcellular location Host nucleus.

Gene Ontology [?]

GO:0006260; Biological process: DNA replication.
GO:0003677; Molecular function: DNA binding.
GO:0042025; Cellular component: host cell nucleus.
case <FTGroup:1>
GO:0046872; Molecular function: metal ion binding.
end case

case <FTTag:act_site>
GO:0016888; Molecular function: endodeoxyribonuclease activity, producing 5'-phosphomonoesters.
GO:0016779; Molecular function: nucleotidyltransferase activity.
end case

Keywords [?]

case <FTTag:act_site>
end case

case <FTGroup:1>
end case

Features [?]

From: PS52020
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     from     to       CRESS-DNA virus Rep endonuclease #        
MOTIF     8     11       RCR-1     [FWILMVACGTSYPH](2)-T-[FWILMVACGTSYPH]  
MOTIF     47     49       RCR-2     H-[FWILMVACGTSYPH]-[HQ]  
MOTIF     87     90       RCR-3     Y-x(2)-K  
ACT_SITE     87     87       For DNA cleavage activity   act_site   Y  
BINDING     39     39       /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240     E   1
BINDING     47     47       /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240     H   1
BINDING     49     49       /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240     H   1
BINDING     91     91       /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240     [DEN]   1

Additional information [?]

Size range 94-130 amino acids
Related rules None
Repeats 1
Topology Undefined
Example D4N3P2 (REP_HCYV5)
Viruses; Cressdnaviricota
Bacteria; Candidatus Phytoplasma asteris
Comments None


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UniProtKB rule member sequences [?]