ProRule PRU01364

General rule information [?]

Accession PRU01364
Dates 17-MAR-2023 (Created)
-- (Last updated, Version 1)
Data class Domain;
Predictors PROSITE; PS52020; CRESS_DNA_REP
Name CRESS-DNA virus replication initiator protein (Rep) endonuclease domain
Function The Rep endonuclease domain is characterized by rolling circle replication (RCR) motifs I through III, which are important for RCR initiation and termination. Motif I, UUTU (U denotes hydrophobic residues), is thought to be involved in the recognition of the origin of replication. Motif II, HU(H/Q), is involved in the coordination of divalent metal ions, Mg(2+) or Mn(2+), which are essential for endonuclease activity at the origin of replication. Motif III (YxxK, where x is any amino acid) is involved in dsDNA cleavage and subsequent covalent attachment of the Rep through the catalytic tyrosine residue to the 5' end of the cleaved product.
Scope(s) Viruses
Candidatus Phytoplasma asteris
Example(s) D4N3P2 (REP_HCYV5);

Propagated annotation [?]

Comments [?]

case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Divalent metal cations, possibly Mg(2+) or Mn(2+).
end case

Keywords [?]

Gene Ontology [?]

GO:0006260; Biological process:DNA replication
GO:0003677; Molecular function:DNA binding
GO:0042025; Cellular component:host cell nucleus
case <FTGroup:1>
GO:0046872; Molecular function:metal ion binding
end case
case <FTTag:act_site>
GO:0016888; Molecular function:endodeoxyribonuclease activity, producing 5'-phosphomonoesters
GO:0016779; Molecular function:nucleotidyltransferase activity
end case

Features [?]

From: PS52020
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="CRESS-DNA virus Rep endonuclease #"
MOTIF 87 90 /note="RCR-3" Y-x(2)-K
ACT_SITE 87 87 /note="For DNA cleavage activity" act_site Y
BINDING 39 39 /ligand="a divalent metal cation #1"
E 1
BINDING 47 47 /ligand="a divalent metal cation #1"
H 1
BINDING 49 49 /ligand="a divalent metal cation #1"
H 1
BINDING 91 91 /ligand="a divalent metal cation #1"
[DEN] 1

Additional information [?]

Size range 94-130 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined


PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

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UniProtKB rule member sequences [?]