PRU01364

General rule information [?]

Accession	PRU01364
Dates	17-MAR-2023 (Created) 17-MAR-2023 (Last updated, Version 1)

Data class

Domain

Predictors

PROSITE; PS52020; CRESS_DNA_REP

Name	CRESS-DNA virus replication initiator protein (Rep) endonuclease domain

Function

The Rep endonuclease domain is characterized by rolling circle replication (RCR) motifs I through III, which are important for RCR initiation and termination. Motif I, UUTU (U denotes hydrophobic residues), is thought to be involved in the recognition of the origin of replication. Motif II, HU(H/Q), is involved in the coordination of divalent metal ions, Mg(2+) or Mn(2+), which are essential for endonuclease activity at the origin of replication. Motif III (YxxK, where x is any amino acid) is involved in dsDNA cleavage and subsequent covalent attachment of the Rep through the catalytic tyrosine residue to the 5' end of the cleaved product.

Propagated annotation [?]

Comments [?]

case <FTGroup:1>

Cofactor

Mg(2+)
Mn(2+)
Note: Divalent metal cations, possibly Mg(2+) or Mn(2+)

end case

Subcellular location

Host nucleus.

Gene Ontology [?]

GO:0006260; Biological process: DNA replication.
GO:0003677; Molecular function: DNA binding.
GO:0042025; Cellular component: host cell nucleus.

case <FTGroup:1>

GO:0046872; Molecular function: metal ion binding.

end case

case <FTTag:act_site>

GO:0016888; Molecular function: endodeoxyribonuclease activity, producing 5'-phosphomonoesters.
GO:0016779; Molecular function: nucleotidyltransferase activity.

end case

Keywords [?]

DNA-binding
Host nucleus
DNA replication
Nucleotide-binding

case <FTTag:act_site>

Endonuclease
Hydrolase
Nuclease
Covalent protein-DNA linkage
Nucleotidyltransferase
Transferase

end case

case <FTGroup:1>

Metal-binding

end case

Features [?]

From: PS52020

Key From To Description Tag Condition FTGroup

DOMAIN from to CRESS-DNA virus Rep endonuclease #

MOTIF 8 11 RCR-1 [FWILMVACGTSYPH](2)-T-[FWILMVACGTSYPH]

MOTIF 47 49 RCR-2 H-[FWILMVACGTSYPH]-[HQ]

MOTIF 87 90 RCR-3 Y-x(2)-K

ACT_SITE 87 87 For DNA cleavage activity act_site Y

BINDING 39 39 /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 E 1

BINDING 47 47 /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 H 1

BINDING 49 49 /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 H 1

BINDING 91 91 /ligand="a divalent metal cation #1" /ligand_id="ChEBI:CHEBI:60240 [DEN] 1

Additional information [?]

Size range	94-130 amino acids
Related rules	None
Repeats	1
Topology	Undefined
Example	D4N3P2 (REP_HCYV5)
Scope	Viruses; Cressdnaviricota Bacteria; Candidatus Phytoplasma asteris
Comments	None

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

View rule in raw text format (no links)

UniProtKB rule member sequences [?]

UniProtKB/Swiss-Prot sets

Bacteria [1]

Viruses [73]

All [ 74 ]
Retrieve set of proteins with 3D structure for this domain

Bacteria	[1]
Viruses	[73]
All	[ 74 ]