ProRule PRU01380

Accession	PRU01380
Dates	13-SEP-2023 (Created) -- (Last updated, Version 1)
Data class	Domain;
Predictors	PROSITE; PS52036; ZF_RG_N
Name	Zinc finger reverse gyrase N-terminal-type
Function	Reverse gyrase (RG) is an ATP-dependent topoisomerase that is only found in archaeal and bacterial hyperthermophiles (above 80 degre C) and thermophiles (65-80 degre C). RGs share a modular structure with an N- terminal cysteine-rich region (a zinc finger) preceding a helicase domain that is followed by a C-terminal topoisomerase domain. The helicase domain is subdivided into H1 and H2 domains that are flexibly linked. Some RG topoisomerase domains also include a cysteine-rich region that has been proposed to form a second zinc finger. The two zinc-finger motifs are found to be important to the structure stability maintenance of RG at high temperature. The N-terminal zinc finger firmly attaches the H1 domain to the topoisomerase domain and may contribute to double-strand DNA (dsDNA) binding.
Scope(s)	Archaea Bacteria
Example(s)	P0DW67 (RGYR2_SULAC);

From: PS52036
Key	From	To	Description	Tag	Condition	FTGroup
case (<Feature:PS52036:11=[C]> and <Feature:PS52036:14=[C]> and <Feature:PS52036:29=[C]> and <Feature:PS52036:32=[C]>)
ZN_FING	from	to	/note="RG N-terminal-type #"
else case (<Feature:PS52036:11=[CH]> and <Feature:PS52036:14=[CH]> and <Feature:PS52036:29=[CH]> and <Feature:PS52036:32=[CH]>)
ZN_FING	from	to	/note="RG N-terminal-type #; atypical"
else
ZN_FING	from	to	/note="RG N-terminal-type #; degenerate"
end case
BINDING	11	11	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1"		[CH]	1
BINDING	14	14	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1"		[CH]	1
BINDING	29	29	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1"		[CH]	1
BINDING	32	32	/ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="#1"		[CH]	1