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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

ProRule PRU01380


View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01380
General rule information [?]

Accession PRU01380
Dates 13-SEP-2023 (Created)
13-SEP-2023 (Last updated, Version 2)
Data class Domain;
Predictors PROSITE; PS52036; ZF_RG_N
Name Zinc finger reverse gyrase N-terminal-type
Function Reverse gyrase (RG) is an ATP-dependent topoisomerase that is only found in archaeal and bacterial hyperthermophiles (above 80 degre C) and thermophiles (65-80 degre C). RGs share a modular structure with an N- terminal cysteine-rich region (a zinc finger) preceding a helicase domain that is followed by a C-terminal topoisomerase domain. The helicase domain is subdivided into H1 and H2 domains that are flexibly linked. Some RG topoisomerase domains also include a cysteine-rich region that has been proposed to form a second zinc finger. The two zinc-finger motifs are found to be important to the structure stability maintenance of RG at high temperature. The N-terminal zinc finger firmly attaches the H1 domain to the topoisomerase domain and may contribute to double-strand DNA (dsDNA) binding.
Scope(s) Archaea
Bacteria
Example(s) P0DW67;

Propagated annotation [?]

Comments [?]

case (<Feature:PS52036:11=[CH]> and <Feature:PS52036:14=[CH]> and <Feature:PS52036:29=[CH]> and <Feature:PS52036:32=[CH]>)
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105;

Keywords [?]

Metal-binding
Zinc
end case
Zinc-finger

Gene Ontology [?]

GO:0046872; Molecular function:metal ion binding
GO:0008270; Molecular function:zinc ion binding

Features [?]

From: PS52036
Key From To Description Tag Condition FTGroup
case (<Feature:PS52036:11=[C]> and <Feature:PS52036:14=[C]> and <Feature:PS52036:29=[C]> and <Feature:PS52036:32=[C]>)
ZN_FING from to /note="RG N-terminal-type #"
else case (<Feature:PS52036:11=[CH]> and <Feature:PS52036:14=[CH]> and <Feature:PS52036:29=[CH]> and <Feature:PS52036:32=[CH]>)
ZN_FING from to /note="RG N-terminal-type #; atypical"
else
ZN_FING from to /note="RG N-terminal-type #; degenerate"
end case
BINDING 11 11 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 1
BINDING 14 14 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 1
BINDING 29 29 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 1
BINDING 32 32 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="#1"		 [CH] 1

Additional information [?]

Size range 37-47 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.

UniProtKB rule member sequences [?]