We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU01382
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01382
General rule information
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| Accession | PRU01382 |
| Dates | 19-SEP-2023 (Created)
19-SEP-2023 (Last updated, Version 2) |
| Data class | Domain; |
| Predictors |
PROSITE; PS52038; TOPO_IB_2 |
Name | Topoisomerase (Topo) IB-type catalytic domain |
| Function | The RKKRH/N "catalytic pentad", conserved in all members of the topo IB family, catalyzes the attack of the active site tyrosine nucleophile on the scissile phosphodiester. |
| Scope(s) |
Eukaryota Viruses Nucleocytoviricota |
| Example(s) | Q8R4U6; |
Propagated annotation
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Identifier, protein and gene names
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| case <FTTag:act_site> | |
| Protein name | + AltName: EC=5.6.2.1; |
Comments
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| CATALYTIC ACTIVITY | Reaction=ATP-independent breakage of single- stranded DNA, followed by passage and rejoining.; EC=5.6.2.1; |
| SIMILARITY | Belongs to the type IB topoisomerase family. |
Keywords
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| DNA-binding | |
| case <FTTag:act_site> | |
| Isomerase | |
| Topoisomerase | |
| end case | |
Gene Ontology
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| GO:0003917; Molecular function:DNA topoisomerase type I (single strand cut, ATP-independent) activity | |
| GO:0006265; Biological process:DNA topological change | |
| end case | |
| GO:0003677; Molecular function:DNA binding | |
Cross-references
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| PROSITE | PS00176; TOPO_IB_1; 1; |
Features
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| From: PS52038 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="Topo IB-type catalytic #" | |||||||||
| ACT_SITE | 262 | 262 | /note="O-(3'-phospho-DNA)-tyrosine intermediate" | act_site | Y | |||||||
Additional information
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| Size range | 238-475 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Eukaryota [21] Viruses [7] All [ 28 ]
- Retrieve set of proteins with 3D structure for this domain