We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
ProRule PRU01393
View rule in raw text format (no links)
PURL: https://purl.expasy.org/prosite/rule/PRU01393
General rule information
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| Accession | PRU01393 |
| Dates | 08-AUG-2024 (Created)
13-SEP-2024 (Last updated, Version 4) |
| Data class | Domain; |
| Predictors |
PROSITE; PS52048; UCH_DOMAIN |
Name | Ubiquitin carboxyl-terminal hydrolase (UCH) catalytic domain |
| Function | Ubiquitin C-terminal hydrolases (UCHs) are deubiquitinating enzymes which hydrolyze C-terminal esters and amides of ubiquitin. They have a catalytic core domain consisting of about 230 amino acids. |
| Scope(s) |
Eukaryota |
| Example(s) | Q6SEG5; |
Propagated annotation
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Identifier, protein and gene names
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| case <FTGroup:1> | |
| Protein name | + RecName: EC=3.4.19.12; |
Comments
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| CATALYTIC ACTIVITY | Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C- terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| end case | |
| SIMILARITY | Belongs to the peptidase C12 family. |
Keywords
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Gene Ontology
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| GO:0004843; Molecular function:cysteine-type deubiquitinase activity |
| GO:0006511; Biological process:ubiquitin-dependent protein catabolic process |
Cross-references
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| PROSITE | PS00140; UCH_1; 0-1; |
Features
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| From: PS52048 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | from | to | /note="UCH catalytic #" | |||||||||
| ACT_SITE | 88 | 88 | /note="Nucleophile" | C | 1 | |||||||
| ACT_SITE | 163 | 163 | /note="Proton donor" | H | 1 | |||||||
| SITE | 82 | 82 | /note="Transition state stabilizer" | Q | 1 | |||||||
| SITE | 178 | 178 | /note="Important for enzyme activity" | D | 1 | |||||||
Additional information
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| Size range | 209-323 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.
UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Eukaryota [53] All [ 53 ]
- Retrieve set of proteins with 3D structure for this domain