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ProRule PRU01449


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PURL: https://purl.expasy.org/prosite/rule/PRU01449
General rule information [?]

Accession PRU01449
Dates 21-MAY-2026 (Created)
21-MAY-2026 (Last updated, Version )
Data class Domain;
Predictors PROSITE; PS52105; HV_L_ENDO
Name Hantaviridae family large (L) protein endonuclease (endoN) domain
Function The endonuclease and cap-binding functions are required for viral transcription initiation via cap-snatching to provide short, capped RNA primers derived from host cell mRNAs.
Scope(s) Viruses
Orthohantavirus
Example(s) P23456 (L_HANTV);

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name Includes:
RecName: Full=cap-snatching endonuclease;
                 EC=3.1.-.-;

Comments [?]

case <FTGroup:1> and <FTGroup:2> and <FTTag:act_site>
COFACTORName=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=For endonuclease activity. Binds 2 Mn(2+) ions in the active site. The divalent metal ions are crucial for catalytic activity.
end case
DOMAINThe N-terminus contains the endonuclease activity (endoN) (By similarity).
MISCELLANEOUSClassified as His(+) endonuclease since it has a histidine upstream of the active site that coordinates the first cation.
SIMILARITYBelongs to the Bunyavirales RNA polymerase family.

Keywords [?]

case <FTGroup:1> and <FTGroup:2> and <FTTag:act_site>
Hydrolase
Nuclease
Endonuclease
end case
case <FTGroup:1> or <FTGroup:2>
Manganese
Metal-binding
end case
Cap snatching
Viral RNA replication

Gene Ontology [?]

case <FTGroup:1> and <FTGroup:2> and <FTTag:act_site>
GO:0016787; Molecular function:hydrolase activity
GO:0004519; Molecular function:endonuclease activity
end case
case <FTGroup:1> or <FTGroup:2>
GO:0046872; Molecular function:metal ion binding
end case
GO:0075526; Biological process:cap snatching
GO:0006351; Biological process:DNA-templated transcription

Features [?]

From: PS52105
Key From To Description Tag Condition FTGroup
DOMAIN from to /note="Cap-snatching endoN Hantaviridae-type #"
ACT_SITE 124 124 /note="For endonuclease activity" act_site K
BINDING 36 36 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="#1"		 H 1
BINDING 54 54 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="#2"		 E 2
BINDING 97 97 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="#1"		 D 1
BINDING 97 97 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="#2"		 D 2
BINDING 110 110 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="#1"		 E 1
BINDING 111 111 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="#1"		 V 1

Additional information [?]

Size range 217-217 amino acids
Related rules None
Fusion None
Repeats 1
Topology Undefined

Copyright

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UniProtKB rule member sequences [?]