annotation rule: PRU00860

General rule information [?]

Accession PRU00860
Dates 9-JUN-2011 (Created)
20-MAR-2017 (Last updated, Version 5)
Data class Domain
Predictors PROSITE; PS51528; FLAVIVIRUS_NS3PRO
Name Flavivirus NS3 protease (NS3pro) domain
Function The NS3pro has a classical serine protease catalytic triad (His, Asp, and Ser). The enzymatic activity of NS3pro is enhanced by interacting with the central 40 amino acid of NS2B which acts as an essential cofactor.

Propagated annotation [?]


Comments [?]

case <FTGroup:1>
Function Serine protease NS3: Displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction.
Subcellular location Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Remains non-covalently associated to serine protease subunit NS2B.
end case


Gene Ontology [?]

case <FTGroup:1>
GO:0016787; Molecular function: hydrolase activity.
GO:0008233; Molecular function: peptidase activity.
GO:0008236; Molecular function: serine-type peptidase activity.

Keywords [?]

end case


Features [?]

From: PS51528
Key     From     To       Description   Tag   Condition   FTGroup
CHAIN     ?     ?       Serine protease NS3        
DOMAIN     from     to       Peptidase S7 #        
ACT_SITE     53     53       Charge relay system; for serine protease NS3 activity     H   1
ACT_SITE     77     77       Charge relay system; for serine protease NS3 activity     D   1
ACT_SITE     137     137       Charge relay system; for serine protease NS3 activity     S   1

Additional information [?]

Size range 168-191 amino acids
Related rules None
Repeats 1
Topology Undefined
Example P07564 (POLG_DEN2J)
Scope Viruses; Flaviviridae
Comments None

Copyright

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UniProtKB rule member sequences [?]