ProRule PRU00860
General rule information
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| Accession | PRU00860 |
| Dates | 9-JUN-2011 (Created)
21-NOV-2019 (Last updated, Version 6) |
| Data class | Domain; |
| Predictors |
PROSITE; PS51528; FLAVIVIRUS_NS3PRO |
Name | Flavivirus NS3 protease (NS3pro) domain |
| Function | The NS3pro has a classical serine protease catalytic triad (His, Asp, and Ser). The enzymatic activity of NS3pro is enhanced by interacting with the central 40 amino acid of NS2B which acts as an essential cofactor. |
| Scope(s) |
Viruses Flaviviridae |
| Example(s) | P07564 (POLG_DEN2J); |
Propagated annotation
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Comments
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| case <FTGroup:1> | |
| FUNCTION | Serine protease NS3: Displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B- NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction. |
| SUBCELLULAR LOCATION | Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Remains non-covalently associated to serine protease subunit NS2B. |
| end case | |
Keywords
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Gene Ontology
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| case <FTGroup:1> | |
| GO:0016787; Molecular function:hydrolase activity | |
| GO:0008233; Molecular function:peptidase activity | |
| GO:0008236; Molecular function:serine-type peptidase activity | |
Features
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| From: PS51528 | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| CHAIN | ? | ? | /note="Serine protease NS3" | |||||||||
| DOMAIN | from | to | /note="Peptidase S7 #" | |||||||||
| ACT_SITE | 53 | 53 | /note="Charge relay system; for serine protease NS3 activity" | H | 1 | |||||||
| ACT_SITE | 77 | 77 | /note="Charge relay system; for serine protease NS3 activity" | D | 1 | |||||||
| ACT_SITE | 137 | 137 | /note="Charge relay system; for serine protease NS3 activity" | S | 1 | |||||||
Additional information
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| Size range | 168-191 amino acids |
| Related rules |
None |
| Fusion | None |
| Repeats | 1 |
| Topology | Undefined |
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UniProtKB rule member sequences
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- UniProtKB/Swiss-Prot sets
Viruses [60] All [ 60 ]
- Retrieve set of proteins with 3D structure for this domain