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PROSITE documentation PDOC00088

N-4 cytosine-specific DNA methylases signature

Description:

N-4 cytosine-specific DNA methylases (EC 2.1.1.113) [1,2,3] are enzymes that specifically methylate the amino group at the C-4 position of cytosines in DNA. Such enzymes are found as components of type II restriction-modification systems in prokaryotes. Such enzymes recognize a specific sequence in DNA and methylate a cytosine in that sequence. By this action they protect DNA from cleavage by type II restriction enzymes that recognize the same sequence.

Type II N-4 Mtases seem to be structurally and enzymatically related to N-6 adenine-specific DNA methylases. Like the N-6 Mtases they contain a conserved Pro-Pro-Tyr/Phe region, but the N- and C-terminal contexts of this region are sufficiently different to derive a consensus pattern specific to this type of enzymes.

Expert(s) to contact by email:

Roberts R.J.
 Bickle T.

Last update:

November 1997 / Text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

N4_MTASE, PS00093; N-4 cytosine-specific DNA methylases signature  (PATTERN)
Consensus pattern: [LIVMF]-T-S-P-P-[FY]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: 5.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00093
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00093
• Scan Swiss-Prot/TrEMBL entries against PS00093
• view ligand binding statistics
Matching PDB structures: 1BOO [ALL]

References:

1 AuthorsTao T., Walter J., Brennan K.J., Cotterman M.M., Blumenthal R.M.
TitleSequence, internal homology and high-level expression of the gene for a DNA-(cytosine N4)-methyltransferase, M.Pvu II.
SourceNucleic Acids Res. 17:4161-4175(1989).
PubMed ID2662138
2 AuthorsKlimasauskas S., Timinskas A., Menkevicius S., Butkiene D., Butkus V., Janulaitis A.
TitleSequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases.
SourceNucleic Acids Res. 17:9823-9832(1989).
PubMed ID2690010
3 AuthorsTiminskas A., Butkus V., Janulaitis A.
TitleSequence motifs characteristic for DNA [cytosine-N4] and DNA [adenine-N6] methyltransferases. Classification of all DNA methyltransferases.
SourceGene 157:3-11(1995).
PubMed ID7607512

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