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PROSITE documentation PDOC00096

Purine/pyrimidine phosphoribosyl transferases signature

Description:

Phosphoribosyltransferases (PRT) are enzymes that catalyze the synthesis of β-n-5'-monophosphates from phosphoribosylpyrophosphate (PRPP) and an enzyme specific amine. A number of PRT's are involved in the biosynthesis of purine, pyrimidine, and pyridine nucleotides, or in the salvage of purines and pyrimidines. These enzymes are:

  • Adenine phosphoribosyltransferase (EC 2.4.2.7) (APRT), which is involved in purine salvage.
  • Hypoxanthine-guanine or hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) (HGPRT or HPRT), which are involved in purine salvage.
  • Orotate phosphoribosyltransferase (EC 2.4.2.10) (OPRT), which is involved in pyrimidine biosynthesis.
  • Amido phosphoribosyltransferase (EC 2.4.2.14), which is involved in purine biosynthesis.
  • Xanthine-guanine phosphoribosyltransferase (EC 2.4.2.22) (XGPRT), which is involved in purine salvage.

In the sequence of all these enzymes there is a small conserved region which may be involved in the enzymatic activity and/or be part of the PRPP binding site [1].

Note:

In position 11 of the pattern most of these enzymes have Gly.

Last update:

November 1997 / Pattern and text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

PUR_PYR_PR_TRANSFER, PS00103; Purine/pyrimidine phosphoribosyl transferases signature  (PATTERN)
Consensus pattern: [LIVMFYWCTA]-[LIVM]-[LIVMA]-[LIVMFC]-[DE]-D-[LIVMS]-[LIVM]-[STAVD]-[STAR]-[GAC]-x-[STAR]
Sequences known to belong to this class detected by the pattern: ALL, except for Bacillus subtilis xanthine phosphoribosyltransferase
Other sequence(s) detected in Swiss-Prot: bacterial phosphoribosyl pyro- phosphate synthases and 7 other proteins.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00103
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00103
• Scan Swiss-Prot/TrEMBL entries against PS00103
• view ligand binding statistics
Matching PDB structures: 1A95 1A96 1A97 1AO0 ... [ALL]

Reference:

1 AuthorsHershey H.V., Taylor M.W.
TitleNucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes.
SourceGene 43:287-293(1986).
PubMed ID3527873

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