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| PROSITE documentation PDOC00167 |
Biotin-requiring enzymes attachment site
Description:
Biotin, which plays a catalytic role in some carboxyl transfer reactions, is
covalently attached, via an amide bond, to a lysine residue in enzymes
requiring this coenzyme [1,2,3,4]. Such enzymes are:
- Pyruvate carboxylase (EC 6.4.1.1).
- Acetyl-CoA carboxylase (EC 6.4.1.2).
- Propionyl-CoA carboxylase (EC 6.4.1.3).
- Methylcrotonyl-CoA carboxylase (EC 6.4.1.4).
- Geranoyl-CoA carboxylase (EC 6.4.1.5).
- Urea carboxylase (EC 6.3.4.6).
- Oxaloacetate decarboxylase (EC 4.1.1.3).
- Methylmalonyl-CoA decarboxylase (EC 4.1.1.41).
- Glutaconyl-CoA decarboxylase (EC 4.1.1.70).
- Methylmalonyl-CoA carboxyl-transferase (EC 2.1.3.1) (transcarboxylase).
Sequence data reveal that the region around the biocytin (biotin-lysine)
residue is well conserved and can be used as a signature pattern.
Note:
The domain around the biotin-binding lysine residue is evolutionary
related to that around the lipoyl-binding lysine residue of 2-oxo acid
dehydrogenase acyltransferases (see <PDOC00168>).
Last update:
December 2001 / Pattern and text revised.
Technical section:
PROSITE method (with tools and information) covered by this documentation:
| BIOTIN, PS00188; Biotin-requiring enzymes attachment site (PATTERN) |
| Consensus pattern: |
[GDN]-[DEQTR]-x-[LIVMFY]-x(2)-[LIVM]-x-[AIV]-M-K-[LVMAT]-x(3)-[LIVM]-x-[SAV]
K is the biotin attachment site |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1A6X 1BDO 1DCZ 1DD2 ... [ALL] |
References:
| 2 |
Authors | Samols D., Thornton C.G., Murtif V.L., Kumar G.K., Haase F.C., Wood H.G. |
| Title | Evolutionary conservation among biotin enzymes. |
| Source | J. Biol. Chem. 263:6461-6464(1988). |
| PubMed ID | 2896195 |
| 3 |
Authors | Goss N.H., Wood H.G. |
| Title | Formation of N epsilon-(biotinyl)lysine in biotin enzymes. |
| Source | Methods Enzymol. 107:261-278(1984). |
| PubMed ID | 6438443 |
| 4 |
Authors | Shenoy B.C., Xie Y., Park V.L., Kumar G.K., Beegen H., Wood H.G., Samols D. |
| Title | The importance of methionine residues for the catalysis of the biotin enzyme, transcarboxylase. Analysis by site-directed mutagenesis. |
| Source | J. Biol. Chem. 267:18407-18412(1992). |
| PubMed ID | 1526981 |
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