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Fructose-bisphosphate aldolase (EC 4.1.2.13) [1,2] is a glycolytic enzyme that catalyzes the reversible aldol cleavage or condensation of fructose-1,6-bisphosphate into dihydroxyacetone-phosphate and glyceraldehyde 3-phosphate. There are two classes of fructose-bisphosphate aldolases with different catalytic mechanisms. Class-II aldolases [2], mainly found in prokaryotes and fungi, are homodimeric enzymes which require a divalent metal ion - generally zinc - for their activity.

This family also includes the following proteins:

• Escherichia coli galactitol operon protein gatY which catalyzes the transformation of tagatose 1,6-bisphosphate into glycerone phosphate and D- glyceraldehyde 3-phosphate.
• Escherichia coli N-acetyl galactosamine operon protein agaY which catalyzes the same reaction as that of gatY.

As signature patterns for this class of enzyme, we selected two conserved regions. The first pattern is located in the first half of the sequence and contains two histidine residues that have been shown [4] to be involved in binding a zinc ion. The second is located in the C-terminal section and contains clustered acidic residues and glycines.

December 2001 / Pattern and text revised.

PROSITE methods (with tools and information) covered by this documentation:

ALDOLASE_CLASS_II_1, PS00602; Fructose-bisphosphate aldolase class-II signature 1  (PATTERN)

Consensus pattern: [FYVMT]-x(1,3)-[LIVMH]-[APNT]-[LIVM]-x(1,2)-[LIVM]-H-x-D-H-[GACH] The two H's are zinc ligands Sequences known to belong to this class detected by the pattern: ALL, except for Mycoplasma pneumoniae aldolase Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00602 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00602 • Scan Swiss-Prot/TrEMBL entries against PS00602 • view ligand binding statistics

Matching PDB structures: 1B57 1DOS 1GVF 1GYN ... [ALL]

ALDOLASE_CLASS_II_2, PS00806; Fructose-bisphosphate aldolase class-II signature 2  (PATTERN)

Consensus pattern: [LIVM]-E-x-E-[LIVM]-G-x(2)-[GM]-[GSTA]-x-E Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00806 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00806 • Scan Swiss-Prot/TrEMBL entries against PS00806 • view ligand binding statistics

Matching PDB structures: 1B57 1DOS 4DEL [ALL]

1	Authors	Perham R.N.
	Title	The fructose-1,6-bisphosphate aldolases: same reaction, different enzymes.
	Source	Biochem. Soc. Trans. 18:185-187(1990).
	PubMed ID	2199259

2	Authors	Marsh J.J., Lebherz H.G.
	Title	Fructose-bisphosphate aldolases: an evolutionary history.
	Source	Trends Biochem. Sci. 17:110-113(1992).
	PubMed ID	1412694

3	Authors	von der Osten C.H., Barbas C.F. III, Wong C.-H., Sinskey A.J.
	Source	Mol. Microbiol. 3:1625-1637(1989).

4	Authors	Berry A., Marshall K.E.
	Title	Identification of zinc-binding ligands in the class II fructose-1,6-bisphosphate aldolase of Escherichia coli.
	Source	FEBS Lett. 318:11-16(1993).
	PubMed ID	8436219

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