PROSITE documentation PDOC00552

Gram-negative pili assembly chaperone signature

Description

Most Gram-negative bacteria possess a supramolecular structure - the pili - on their surface, that mediates attachment to specific receptors. The assembly of the pili requires the help of specialized periplasmic chaperones [1,2,3]. These proteins are structurally and possibly evolutionary related to the immunoglobulin family. Known members of this family are listed below:

  • Escherichia coli papD for P pili.
  • Escherichia coli afaB for AFA-III adhesin.
  • Escherichia coli aggD for AAF/I fimbriae.
  • Escherichia coli cssC for CS6 antigen.
  • Escherichia coli F17-D for F17 pili.
  • Escherichia coli faeE for K88 pili.
  • Escherichia coli fanE for K99 pili.
  • Escherichia coli fimC for type 1 fimbriae.
  • Escherichia coli focC for F1C fimbriae.
  • Escherichia coli nfaE for NFA-I adhesin.
  • Escherichia coli sfaE for S pili.
  • Escherichia coli clpE for CS31A capsule-like antigen.
  • Escherichia coli ecpD for a yet unknown pilin.
  • Escherichia coli CS3-1 for CS3 pili.
  • Bordetella pertussis fhaD (or fimB) for filamentous hemagglutinin and fimbria.
  • Haemophilus influenzae hifB for type b pili.
  • Klebsiella pneumoniae mrkB for type 3 pili.
  • Proteus mirabilis pmfD for PMF fimbriae.
  • Salmonella enteritidis sefB for sef14 fimbriae.
  • Salmonella typhimurium lpfB for long polar fimbriae.
  • Yersinia enterocolitica myfB for myf fimbriae.
  • Yersinia pestis caf1M for F1 capsule antigen.
  • Yersinia pestis psaB for pH6 antigen.
  • Escherichia coli hypothetical protein ybgP.
  • Escherichia coli hypothetical protein ycbF.
  • Escherichia coli hypothetical protein yehC.
  • Escherichia coli hypothetical protein yfcS.
  • Escherichia coli hypothetical protein yhcA.
  • Escherichia coli hypothetical protein yqiH.
  • Escherichia coli hypothetical protein yraI.

Structurally, these proteins consist of two globular domains with a topology identical to an immunoglobulin fold. As a signature pattern, we selected a highly conserved region located in the C-terminal section of the first globular domain.

Last update:

December 2004 / Pattern and text revised.

Technical section

PROSITE method (with tools and information) covered by this documentation:

PILI_CHAPERONE, PS00635; Gram-negative pili assembly chaperone signature  (PATTERN)
Consensus pattern: [LIVMFY]-[APN]-x-[DNS]-[KREQ]-E-[STR]-[LIVMAR]-x-[FYWTE]-x-[NCS]-[LIVM]-x(2)-[LIVM]-P-[PAS]
Sequences known to belong to this class detected by the pattern: ALL, except for ycbF and yhcA
Other sequence(s) detected in Swiss-Prot: NONE
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00635
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00635
• Scan Swiss-Prot/TrEMBL entries against PS00635
• view ligand binding statistics
Matching PDB structures: 1BF8 1KIU 1KLF 1L4I ... [ALL]

References

1 Authors Hung D.L., Knight S.D., Woods R.M., Pinkner J.S., Hultgren S.J.
Title Molecular basis of two subfamilies of immunoglobulin-like chaperones.
Source EMBO J. 15:3792-3805(1996).
PubMed ID 8670884
2 Authors Jacob-Dubuisson F., Kuehn M.J., Hultgren S.J.
Source Trends Microbiol. 1:50-55(1993).
3 Authors van Rosmalen M., Saier M.H. Jr.
Source Res. Microbiol. 144:507-527(1993).

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