|PROSITE documentation PDOC00558|
Ribonuclease P (EC 126.96.36.199) (RNase P) [1,2,3] is a site specific endonuclease that generates mature tRNAs by cleaving-off the leader sequences at their 5'ends. In bacteria RNase P is known to be composed of two components: a large (about 400 base pairs) RNA (gene rnpB) and a small protein (119 to 133 amino acids) (gene rnpA). The RNA moiety of RNase P carries the catalytic activity; the function of the protein component is not yet clear although it may act as an electrostatic screen allowing the highly negatively charged RNA enzyme-substrate complex to fold into the catalytic conformation.
The sequence of rnpA is not highly conserved, however there is, in the central part of the protein, a conserved basic region. We have used this region as a signature pattern.Last update:
December 2004 / Pattern and text revised.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Pace N.R., Smith D.|
|Title||Ribonuclease P: function and variation.|
|Source||J. Biol. Chem. 265:3587-3590(1990).|
|Title||Ribonuclease P. Postscript.|
|Source||J. Biol. Chem. 265:20053-20056(1990).|
|3||Authors||Brown J.W., Pace N.R.|
|Title||Ribonuclease P RNA and protein subunits from bacteria.|
|Source||Nucleic Acids Res. 20:1451-1456(1992).|