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PROSITE documentation PDOC00565Glycosyl hydrolases family 5 signature
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PURL: https://purl.expasy.org/prosite/documentation/PDOC00565
The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family A [3] or as the glycosyl hydrolases family 5 [4,E1]. The enzymes which are currently known to belong to this family are listed below.
- Endoglucanases from various species and strains of Bacillus.
- Butyrivibrio fibrisolvens endoglucanases 1 (end1) and A (celA).
- Caldocellum saccharolyticum bifunctional endoglucanase/exoglucanase (celB). This protein consists of two domains; it is the C-terminal domain, which has endoglucanase activity, which belongs to this family.
- Clostridium acetobutylicum endoglucanase (eglA).
- Clostridium cellulolyticum endoglucanases A (celccA) and D (celccD).
- Clostridium cellulovorans endoglucanase B (engB) and D (engD).
- Clostridium thermocellum endoglucanases B (celB), C (celC), E (celE), G (celG) and H (celH).
- Erwinia chrysanthemi endoglucanase Z (celZ).
- Fibrobacter succinogenes endoglucanase 3 (cel-3).
- Pseudomonas fluorescens endoglucanase C (celC).
- Pseudomonas solanacearum endoglucanase (egl).
- Robillarda strain Y-20 endoglucanase I.
- Ruminococcus albus endoglucanases I (EG-I), A (celA), and B (celB).
- Ruminococcus flavefaciens cellodextrinase A (celA).
- Ruminococcus flavefaciens endoglucanase E (celE).
- Streptomyces lividans endoglucanase.
- Thermomonospora fusca endoglucanase E-5 (celE).
- Trichoderma reesei endoglucanase II (EGLII).
- Xanthomonas campestris endoglucanase (engxcA).
As well as:
- Baker's yeast glucan 1,3-β-glucosidase I/II (EC 3.2.1.58) (EXG1).
- Baker's yeast glucan 1,3-β-glucosidase 2 (EC 3.2.1.58) (EXG2).
- Baker's yeast sporulation-specific glucan 1,3-β-glucosidase (SPR1).
- Caldocellum saccharolyticum β-mannanase (EC 3.2.1.78) (manA).
- Yeast hypothetical protein YBR056w.
- Yeast hypothetical protein YIR007w.
One of the conserved regions in these enzymes contains a conserved glutamic acid residue which is potentially involved [5] in the catalytic mechanism. We use this region as a signature pattern.
Expert(s) to contact by email: Last update:December 2004 / Pattern and text revised.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Beguin P. |
| Title | Molecular biology of cellulose degradation. | |
| Source | Annu. Rev. Microbiol. 44:219-248(1990). | |
| PubMed ID | 2252383 | |
| DOI | 10.1146/annurev.mi.44.100190.001251 |
| 2 | Authors | Gilkes N.R. Henrissat B. Kilburn D.G. Miller R.C. Jr. Warren R.A.J. |
| Title | Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. | |
| Source | Microbiol. Rev. 55:303-315(1991). | |
| PubMed ID | 1886523 |
| 3 | Authors | Henrissat B. Claeyssens M. Tomme P. Lemesle L. Mornon J.-P. |
| Title | Cellulase families revealed by hydrophobic cluster analysis. | |
| Source | Gene 81:83-95(1989). | |
| PubMed ID | 2806912 |
| 4 | Authors | Henrissat B. |
| Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. | |
| Source | Biochem. J. 280:309-316(1991). | |
| PubMed ID | 1747104 |
| 5 | Authors | Py B. Bortoli-German I. Haiech J. Chippaux M. Barras F. |
| Title | Cellulase EGZ of Erwinia chrysanthemi: structural organization and importance of His98 and Glu133 residues for catalysis. | |
| Source | Protein Eng. 4:325-333(1991). | |
| PubMed ID | 1677466 |
| E1 | Title | https://www.uniprot.org/docs/glycosid |
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