|PROSITE documentation PDOC00565
Glycosyl hydrolases family 5 signature
The microbial degradation of cellulose and xylans requires several types of
enzymes such as endoglucanases (EC 188.8.131.52), cellobiohydrolases (EC 184.108.40.206)
(exoglucanases), or xylanases (EC 220.127.116.11) [1,2]. Fungi and bacteria produces
a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the
basis of sequence similarities, can be classified into families. One of these
families is known as the cellulase family A  or as the glycosyl hydrolases
family 5 [4,E1]. The enzymes which are currently known to belong to this
family are listed below.
- Endoglucanases from various species and strains of Bacillus.
- Butyrivibrio fibrisolvens endoglucanases 1 (end1) and A (celA).
- Caldocellum saccharolyticum bifunctional endoglucanase/exoglucanase (celB).
This protein consists of two domains; it is the C-terminal domain, which
has endoglucanase activity, which belongs to this family.
- Clostridium acetobutylicum endoglucanase (eglA).
- Clostridium cellulolyticum endoglucanases A (celccA) and D (celccD).
- Clostridium cellulovorans endoglucanase B (engB) and D (engD).
- Clostridium thermocellum endoglucanases B (celB), C (celC), E (celE), G
(celG) and H (celH).
- Erwinia chrysanthemi endoglucanase Z (celZ).
- Fibrobacter succinogenes endoglucanase 3 (cel-3).
- Pseudomonas fluorescens endoglucanase C (celC).
- Pseudomonas solanacearum endoglucanase (egl).
- Robillarda strain Y-20 endoglucanase I.
- Ruminococcus albus endoglucanases I (EG-I), A (celA), and B (celB).
- Ruminococcus flavefaciens cellodextrinase A (celA).
- Ruminococcus flavefaciens endoglucanase E (celE).
- Streptomyces lividans endoglucanase.
- Thermomonospora fusca endoglucanase E-5 (celE).
- Trichoderma reesei endoglucanase II (EGLII).
- Xanthomonas campestris endoglucanase (engxcA).
As well as:
- Baker's yeast glucan 1,3-β-glucosidase I/II (EC 18.104.22.168) (EXG1).
- Baker's yeast glucan 1,3-β-glucosidase 2 (EC 22.214.171.124) (EXG2).
- Baker's yeast sporulation-specific glucan 1,3-β-glucosidase (SPR1).
- Caldocellum saccharolyticum β-mannanase (EC 126.96.36.199) (manA).
- Yeast hypothetical protein YBR056w.
- Yeast hypothetical protein YIR007w.
One of the conserved regions in these enzymes contains a conserved glutamic
acid residue which is potentially involved  in the catalytic mechanism.
We use this region as a signature pattern.
December 2004 / Pattern and text revised.
PROSITE method (with tools and information) covered by this documentation:
|GLYCOSYL_HYDROL_F5, PS00659; Glycosyl hydrolases family 5 signature (PATTERN)
E may be an active site residue
|Sequences known to belong to this class detected by the pattern:
||ALL, except for Robillarda Y-20 endoglucanase I whose sequence is known to be incorrect and yeast YBR056w
|Other sequence(s) detected in Swiss-Prot:
|Matching PDB structures:
1A3H 1CEC 1CZ1 1E5J ... [ALL]
||Gilkes N.R., Henrissat B., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
||Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
||Microbiol. Rev. 55:303-315(1991).
||Henrissat B., Claeyssens M., Tomme P., Lemesle L., Mornon J.-P.
||Cellulase families revealed by hydrophobic cluster analysis.
||A classification of glycosyl hydrolases based on amino acid sequence similarities.
||Biochem. J. 280:309-316(1991).
||Py B., Bortoli-German I., Haiech J., Chippaux M., Barras F.
||Cellulase EGZ of Erwinia chrysanthemi: structural organization and importance of His98 and Glu133 residues for catalysis.
||Protein Eng. 4:325-333(1991).
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