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PROSITE documentation PDOC00565

Glycosyl hydrolases family 5 signature

Description:

The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family A [3] or as the glycosyl hydrolases family 5 [4,E1]. The enzymes which are currently known to belong to this family are listed below.

  • Endoglucanases from various species and strains of Bacillus.
  • Butyrivibrio fibrisolvens endoglucanases 1 (end1) and A (celA).
  • Caldocellum saccharolyticum bifunctional endoglucanase/exoglucanase (celB). This protein consists of two domains; it is the C-terminal domain, which has endoglucanase activity, which belongs to this family.
  • Clostridium acetobutylicum endoglucanase (eglA).
  • Clostridium cellulolyticum endoglucanases A (celccA) and D (celccD).
  • Clostridium cellulovorans endoglucanase B (engB) and D (engD).
  • Clostridium thermocellum endoglucanases B (celB), C (celC), E (celE), G (celG) and H (celH).
  • Erwinia chrysanthemi endoglucanase Z (celZ).
  • Fibrobacter succinogenes endoglucanase 3 (cel-3).
  • Pseudomonas fluorescens endoglucanase C (celC).
  • Pseudomonas solanacearum endoglucanase (egl).
  • Robillarda strain Y-20 endoglucanase I.
  • Ruminococcus albus endoglucanases I (EG-I), A (celA), and B (celB).
  • Ruminococcus flavefaciens cellodextrinase A (celA).
  • Ruminococcus flavefaciens endoglucanase E (celE).
  • Streptomyces lividans endoglucanase.
  • Thermomonospora fusca endoglucanase E-5 (celE).
  • Trichoderma reesei endoglucanase II (EGLII).
  • Xanthomonas campestris endoglucanase (engxcA).

As well as:

  • Baker's yeast glucan 1,3-β-glucosidase I/II (EC 3.2.1.58) (EXG1).
  • Baker's yeast glucan 1,3-β-glucosidase 2 (EC 3.2.1.58) (EXG2).
  • Baker's yeast sporulation-specific glucan 1,3-β-glucosidase (SPR1).
  • Caldocellum saccharolyticum β-mannanase (EC 3.2.1.78) (manA).
  • Yeast hypothetical protein YBR056w.
  • Yeast hypothetical protein YIR007w.

One of the conserved regions in these enzymes contains a conserved glutamic acid residue which is potentially involved [5] in the catalytic mechanism. We use this region as a signature pattern.

Expert(s) to contact by email:

Henrissat B.

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

GLYCOSYL_HYDROL_F5, PS00659; Glycosyl hydrolases family 5 signature  (PATTERN)
Consensus pattern: [LIV]-[LIVMFYWGA](2)-[DNEQG]-[LIVMGST]-{SENR}-N-E-[PV]-[RHDNSTLIVFY]
E may be an active site residue
Sequences known to belong to this class detected by the pattern: ALL, except for Robillarda Y-20 endoglucanase I whose sequence is known to be incorrect and yeast YBR056w
Other sequence(s) detected in Swiss-Prot: 28.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00659
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00659
• Scan Swiss-Prot/TrEMBL entries against PS00659
• view ligand binding statistics
Matching PDB structures: 1A3H 1CEC 1CZ1 1E5J ... [ALL]

References:

1 AuthorsBeguin P.
TitleMolecular biology of cellulose degradation.
SourceAnnu. Rev. Microbiol. 44:219-248(1990).
PubMed ID2252383
DOI10.1146/annurev.mi.44.100190.001251
2 AuthorsGilkes N.R., Henrissat B., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
TitleDomains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
SourceMicrobiol. Rev. 55:303-315(1991).
PubMed ID1886523
3 AuthorsHenrissat B., Claeyssens M., Tomme P., Lemesle L., Mornon J.-P.
TitleCellulase families revealed by hydrophobic cluster analysis.
SourceGene 81:83-95(1989).
PubMed ID2806912
4 AuthorsHenrissat B.
TitleA classification of glycosyl hydrolases based on amino acid sequence similarities.
SourceBiochem. J. 280:309-316(1991).
PubMed ID1747104
5 AuthorsPy B., Bortoli-German I., Haiech J., Chippaux M., Barras F.
TitleCellulase EGZ of Erwinia chrysanthemi: structural organization and importance of His98 and Glu133 residues for catalysis.
SourceProtein Eng. 4:325-333(1991).
PubMed ID1677466
E1
Sourcehttp://www.expasy.org/cgi-bin/lists?glycosid.txt

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