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| PROSITE documentation PDOC00569 |
Dihydrodipicolinate synthase signatures
Description:
Dihydrodipicolinate synthase (EC 4.2.1.52) (DHDPS) [1] catalyzes, in higher
plants chloroplast and in many bacteria (gene dapA), the first reaction
specific to the biosynthesis of lysine and of diaminopimelate. DHDPS is
responsible for the condensation of aspartate semialdehyde and pyruvate by a
ping-pong mechanism in which pyruvate first binds to the enzyme by forming a
Schiff-base with a lysine residue.
Three other proteins are structurally related to DHDPS and probably also act
via a similar catalytic mechanism:
- Escherichia coli N-acetylneuraminate lyase (EC 4.1.3.3) (gene nanA), which
catalyzes the condensation of N-acetyl-D-mannosamine and pyruvate to form
N-acetylneuraminate.
- Rhizobium meliloti protein mosA [3], which is involved in the biosynthesis
of the rhizopine 3-o-methyl-scyllo-inosamine.
- Escherichia coli hypothetical protein yjhH.
We have developed two signature patterns for these enzymes. The first one is
centered on highly conserved region in the N-terminal part of these proteins.
The second signature contains a lysine residue which has been shown, in
Escherichia coli dapA [2], to be the one that forms a Schiff-base with the
substrate.
Last update:
December 2004 / Pattern and text revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| DHDPS_1, PS00665; Dihydrodipicolinate synthase signature 1 (PATTERN) |
| Consensus pattern: |
[GSA]-[LIVM]-[LIVMFY]-x(2)-G-[ST]-[TG]-G-E-[GASNF]-x(6)-[EQ]
|
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1DHP 1F5Z 1F6K 1F6P ... [ALL] |
| DHDPS_2, PS00666; Dihydrodipicolinate synthase signature 2 (PATTERN) |
| Consensus pattern: |
Y-[DNSAH]-[LIVMFAN]-P-x(2)-[STAV]-x(2,3)-[LIVMFT]-x(13,14)-[LIVMCF]-x-[SGA]-[LIVMFNS]-K-[DEQAFYH]-[STACI]
K is involved in Schiff-base formation |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1DHP 1F5Z 1F6K 1F6P ... [ALL] |
References:
| 1 |
Authors | Kaneko T., Hashimoto T., Kumpaisal R., Yamada Y. |
| Title | Molecular cloning of wheat dihydrodipicolinate synthase. |
| Source | J. Biol. Chem. 265:17451-17455(1990). |
| PubMed ID | 2211639 |
| 2 |
Authors | Laber B., Gomis-Ruth F.-X., Romao M.J., Huber R. |
| Title | Escherichia coli dihydrodipicolinate synthase. Identification of the active site and crystallization. |
| Source | Biochem. J. 288:691-695(1992). |
| PubMed ID | 1463470 |
| 3 |
Authors | Murphy P.J., Trenz S.P., Grzemski W., De Bruijn F.J., Schell J. |
| Title | The Rhizobium meliloti rhizopine mos locus is a mosaic structure facilitating its symbiotic regulation. |
| Source | J. Bacteriol. 175:5193-5204(1993). |
| PubMed ID | 8349559 |
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