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| PROSITE documentation PDOC00575 |
Methionine aminopeptidase signatures
Description:
Methionine aminopeptidase (EC 3.4.11.18) (MAP) is responsible for the removal
of the amino-terminal (initiator) methionine from nascent eukaryotic cytosolic
and cytoplasmic prokaryotic proteins if the penultimate amino acid is small
and uncharged. All MAP studied to date are monomeric proteins that require
cobalt ions for activity.
Two subfamilies of MAP enzymes are known to exist [1,2]. While being
evolutionary related, they only share a limited amount of sequence similarity
mostly clustered around the residues shown, in the Escherichia coli MAP [3],
to be involved in cobalt-binding.
The first family consists of enzymes from prokaryotes as well as eukaryotic
MAP-1, while the second group is made up of archebacterial MAP and eukaryotic
MAP-2. The second subfamily also includes proteins which do not seem to be
MAP, but that are clearly evolutionary related such as mouse proliferation-associated protein 1 and fission yeast curved DNA-binding protein.
For each of these subfamilies, we developed a specific signature pattern that
includes residues known to be involved in colbalt-binding.
Note:
These proteins belong to family M24A and M24C in the classification of
peptidases [4,E1].
Last update:
April 2006 / Patterns revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| MAP_1, PS00680; Methionine aminopeptidase subfamily 1 signature (PATTERN) |
| Consensus pattern: |
[MFY]-x-G-H-G-[LIVMC]-[GSHN]-x(3)-H-x(4)-[LIVM]-x(1,2)-[HN]-[YWVHF]
H is a cobalt ligand |
| Sequences known to belong to this class detected by the pattern: |
ALL MAP from family 1 |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1C21 1C22 1C23 1C24 ... [ALL] |
| MAP_2, PS01202; Methionine aminopeptidase subfamily 2 signature (PATTERN) |
| Consensus pattern: |
[DA]-[LIVMY]-x-K-[LIVM]-D-x-G-x-[HQ]-[LIVMS]-[DNS]-G-x(3)-[DN]
The second D and the last D/N are cobalt ligands |
| Sequences known to belong to this class detected by the pattern: |
ALL MAP from family 2. Does not detect fission yeast curved DNA-binding protein |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1B59 1B6A 1BN5 1BOA ... [ALL] |
References:
| 1 |
Authors | Arfin S.M., Kendall R.L., Hall L., Weaver L.H., Stewart A.E., Matthews B.W., Bradshaw R.A. |
| Title | Eukaryotic methionyl aminopeptidases: two classes of cobalt-dependent enzymes. |
| Source | Proc. Natl. Acad. Sci. U.S.A. 92:7714-7718(1995). |
| PubMed ID | 7644482 |
| 2 |
Authors | Keeling P.J., Doolittle W.F. |
| Title | Methionine aminopeptidase-1: the MAP of the mitochondrion? |
| Source | Trends Biochem. Sci. 21:285-286(1996). |
| PubMed ID | 8772380 |
| 3 |
Authors | Roderick S.L., Matthews B.W. |
| Title | Structure of the cobalt-dependent methionine aminopeptidase from Escherichia coli: a new type of proteolytic enzyme. |
| Source | Biochemistry 32:3907-3912(1993). |
| PubMed ID | 8471602 |
| 4 |
Authors | Rawlings N.D., Barrett A.J. |
| Title | Evolutionary families of metallopeptidases. |
| Source | Methods Enzymol. 248:183-228(1995). |
| PubMed ID | 7674922 |
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