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| PROSITE documentation PDOC00621 |
Glycosyl hydrolases family 3 active site
Description:
It has been shown [1,2] that the following glycosyl hydrolases can be, on the
basis of sequence similarities, classified into a single family:
- β glucosidases (EC 3.2.1.21) from the fungi Aspergillus wentii (A-3),
Hansenula anomala, Kluyveromyces fragilis, Saccharomycopsis fibuligera,
(BGL1 and BGL2), Schizophyllum commune and Trichoderma reesei (BGL1).
- β glucosidases from the bacteria Agrobacterium tumefaciens (Cbg1),
Butyrivibrio fibrisolvens (bglA), Clostridium thermocellum (bglB),
Escherichia coli (bglX), Erwinia chrysanthemi (bgxA) and Ruminococcus
albus.
- Alteromonas strain O-7 β-hexosaminidase A (EC 3.2.1.52).
- Bacillus subtilis hypothetical protein yzbA.
- Escherichica coli hypothetical protein ycfO and HI0959, the corresponding
Haemophilus influenzae protein.
One of the conserved regions in these enzymes is centered on a conserved
aspartic acid residue which has been shown [3], in Aspergillus wentii β-glucosidase A3, to be implicated in the catalytic mechanism. We have used this
region as a signature pattern.
Expert(s) to contact by email:
Henrissat B.
Last update:
April 2006 / Pattern revised.
Technical section:
PROSITE method (with tools and information) covered by this documentation:
| GLYCOSYL_HYDROL_F3, PS00775; Glycosyl hydrolases family 3 active site (PATTERN) |
| Consensus pattern: |
[LIVM](2)-[KR]-x-[EQKRD]-x(4)-G-[LIVMFTC]-[LIVT]-[LIVMF]-[ST]-D-x(2)-[SGADNIT]
D is the active site residue |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1TR9 1Y65 2OXN 3BMX ... [ALL] |
References:
| 1 |
Authors | Henrissat B. |
| Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. |
| Source | Biochem. J. 280:309-316(1991). |
| PubMed ID | 1747104 |
| 2 |
Authors | Castle L.A., Smith K.D., Morris R.O. |
| Title | Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase gene involved in modifying a vir-inducing plant signal molecule. |
| Source | J. Bacteriol. 174:1478-1486(1992). |
| PubMed ID | 1537792 |
| 3 |
Authors | Bause E., Legler G. |
| Title | Isolation and structure of a tryptic glycopeptide from the active site of beta-glucosidase A3 from Aspergillus wentii. |
| Source | Biochim. Biophys. Acta 626:459-465(1980). |
| PubMed ID | 6783081 |
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