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PROSITE documentation PDOC00697

3-hydroxyisobutyrate dehydrogenase signature

Description:

3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) catalyzes the NAD-dependent, reversible oxidation of 3-hydroxbutyrate to methylmalonate [1]. In eukaryotes, it is a homodimeric mitochondrial protein involved in valine catabolism. In Pseudomonas aeruginosa [2] (gene mmsB), it is involved in the distal valine metabolic pathway.

The sequence of 3-hydroxyisobutyrate dehydrogenase from eukaryotic and prokaryotic sources show that this enzyme has been well conserved throughout evolution. The following proteins are evolutionary related to 3-hydroxyisobut-yrate dehydrogenase:

  • Escherichia coli 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60) (genes garR and glxR).
  • Escherichia coli hypothetical protein ygbJ.
  • Escherichia coli hypothetical protein yihU.
  • Bacillus subtilis hypothetical protein yfjR.
  • Bacillus subtilis hypothetical protein ykwC.
  • Mycobacterium tuberculosis hypothetical protein Rv0770.
  • Synechocystis strain PCC 6803 hypothetical protein slr0229.

As a signature pattern, we selected a highly conserved glycine-rich region located at the proteins' N-terminus. This region is probably involved in binding NAD.

Last update:

December 2001 / Pattern and text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

3_HYDROXYISOBUT_DH, PS00895; 3-hydroxyisobutyrate dehydrogenase signature  (PATTERN)
Consensus pattern: [LIVMFY](2)-G-L-G-x-[MQ]-G-x(2)-[MA]-[SAV]-x-[SNHR]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE
• Retrieve an alignment of Swiss-Prot true positive hits:
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• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00895
• Scan Swiss-Prot/TrEMBL entries against PS00895
• view ligand binding statistics
Matching PDB structures: 2GF2 2I9P 3CKY [ALL]

References:

1 AuthorsRougraff P.M., Zhang B., Kuntz M.J., Harris R.A., Crabb D.W.
TitleCloning and sequence analysis of a cDNA for 3-hydroxyisobutyrate dehydrogenase. Evidence for its evolutionary relationship to other pyridine nucleotide-dependent dehydrogenases.
SourceJ. Biol. Chem. 264:5899-5903(1989).
PubMed ID2647728
2 AuthorsSteele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R.
TitleCharacterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase.
SourceJ. Biol. Chem. 267:13585-13592(1992).
PubMed ID1339433

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