PROSITE documentation PDOC00701

Glutamate 5-kinase signature




Description

Glutamate 5-kinase (EC 2.7.2.11) (γ-glutamyl kinase) (GK) is the enzyme that catalyzes the first step in the biosynthesis of proline from glutamate, the ATP-dependent phosphorylation of L-glutamate into L-glutamate 5-phosphate.

In eubacteria (gene proB) and yeast [1] (gene PRO1), GK is a monofunctional protein, while in plants and mammals, it is a bifunctional enzyme (P5CS) [2] that consists of two domains: a N-terminal GK domain and a C-terminal γ-glutamyl phosphate reductase domain (EC 1.2.1.41) (see <PDOC00940>).

As a signature pattern, we selected a highly conserved glycine-and alanine-rich region located in the central section of these enzymes.

Yeast hypothetical protein YHR033w is highly similar to GK.

Last update:

December 2004 / Pattern and text revised.

Technical section

PROSITE method (with tools and information) covered by this documentation:

GLUTAMATE_5_KINASE, PS00902; Glutamate 5-kinase signature  (PATTERN)


References

1AuthorsLi W., Brandriss M.C.
TitleProline biosynthesis in Saccharomyces cerevisiae: molecular analysis of the PRO1 gene, which encodes gamma-glutamyl kinase.
SourceJ. Bacteriol. 174:4148-4156(1992).
PubMed ID1350780

2AuthorsHu C.-A.A., Delauney A.J., Verma D.P.S.
TitleA bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants.
SourceProc. Natl. Acad. Sci. U.S.A. 89:9354-9358(1992).
PubMed ID1384052



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