|PROSITE documentation PDOC00794
Glycosyl hydrolases family 16 signature
It has been shown  that the following glycosyl hydrolases can be classified
into a single family on the basis of sequence similarities:
- Bacterial β-1,3-1,4-glucanases, or lichenases, (EC 220.127.116.11) mainly from
Bacillus but also from Clostridium thermocellum (gene licB), Fibrobacter
succinogenes and Rhodothermus marinus (gene bglA).
- Bacillus circulans β-1,3-glucanase A1 (EC 18.104.22.168) (gene glcA).
- Streptomyces coelicolor agarase (EC 22.214.171.124) (gene dagA).
- Alteromonas carrageenovora kappa-carrageenase (EC 126.96.36.199) (gene cgkA).
- Rhizobium meliloti endo-1,3-1,4-β-glycanase exoK.
Two closely clustered conserved glutamates have been shown  to be involved
in the catalytic activity of Bacillus licheniformis lichenase. We used the
region that contains these residues as a signature pattern.
May 2004 / Text revised.
PROSITE method (with tools and information) covered by this documentation:
|GLYCOSYL_HYDROL_F16, PS01034; Glycosyl hydrolases family 16 active sites (PATTERN)
The 2 E's are active site residues
|Sequences known to belong to this class detected by the pattern:
|Other sequence(s) detected in Swiss-Prot:
|Matching PDB structures:
1AJK 1AJO 1AXK 1BYH ... [ALL]
||A classification of glycosyl hydrolases based on amino acid sequence similarities.
||Biochem. J. 280:309-316(1991).
||Juncosa M., Pons J., Dot T., Querol E., Planas A.
||Identification of active site carboxylic residues in Bacillus licheniformis 1,3-1,4-beta-D-glucan 4-glucanohydrolase by site-directed mutagenesis.
||J. Biol. Chem. 269:14530-14535(1994).
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