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The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family K or as the glycosyl hydrolases family 45 [3,E1]. The enzymes which are currently known to belong to this family are listed below.

• Endoglucanase 5 from Humicola insolens.
• Endoglucanase 5 from Trichoderma reesei (egl5).
• Endoglucanase K from Fusarium oxysporum.
• Endoglucanase B from Pseudomonas fluorescens (celB).
• Endoglucanase 1 from Ustilago maydis (egl1).

The best conserved regions in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown [4] to act as a nucleophile in the catalytic mechanism. We use this region as a signature pattern.

Henrissat B.

November 1997 / Pattern and text revised.

PROSITE method (with tools and information) covered by this documentation:

GLYCOSYL_HYDROL_F45, PS01140; Glycosyl hydrolases family 45 active site  (PATTERN)

Consensus pattern: [STA]-T-R-Y-[FYW]-D-x(5)-[CA] The D is an active site residue Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01140 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01140 • Scan Swiss-Prot/TrEMBL entries against PS01140 • view ligand binding statistics

Matching PDB structures: 1HD5 2ENG 3ENG [ALL]

1	Authors	Beguin P.
	Title	Molecular biology of cellulose degradation.
	Source	Annu. Rev. Microbiol. 44:219-248(1990).
	PubMed ID	2252383
	DOI	10.1146/annurev.mi.44.100190.001251

2	Authors	Gilkes N.R., Henrissat B., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
	Title	Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
	Source	Microbiol. Rev. 55:303-315(1991).
	PubMed ID	1886523

3	Authors	Henrissat B., Bairoch A.
	Title	New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.
	Source	Biochem. J. 293:781-788(1993).
	PubMed ID	8352747

4	Authors	Davies G.J., Dodson G.G., Hubbard R.E., Tolley S.P., Dauter Z., Wilson K.S., Hjort C., Mikkelsen J.M., Rasmussen G., Schuelein M.
	Title	Structure and function of endoglucanase V.
	Source	Nature 365:362-364(1993).
	PubMed ID	8377830
	DOI	10.1038/365362a0

E1
Source	http://www.uniprot.org/docs/glycosid

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