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PROSITE documentation PDOC00877

Glycosyl hydrolases family 45 active site

Description:

The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family K or as the glycosyl hydrolases family 45 [3,E1]. The enzymes which are currently known to belong to this family are listed below.

  • Endoglucanase 5 from Humicola insolens.
  • Endoglucanase 5 from Trichoderma reesei (egl5).
  • Endoglucanase K from Fusarium oxysporum.
  • Endoglucanase B from Pseudomonas fluorescens (celB).
  • Endoglucanase 1 from Ustilago maydis (egl1).

The best conserved regions in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown [4] to act as a nucleophile in the catalytic mechanism. We use this region as a signature pattern.

Expert(s) to contact by email:

Henrissat B.

Last update:

November 1997 / Pattern and text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

GLYCOSYL_HYDROL_F45, PS01140; Glycosyl hydrolases family 45 active site  (PATTERN)
Consensus pattern: [STA]-T-R-Y-[FYW]-D-x(5)-[CA]
The D is an active site residue
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01140
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01140
• Scan Swiss-Prot/TrEMBL entries against PS01140
• view ligand binding statistics
Matching PDB structures: 1HD5 2ENG 3ENG [ALL]

References:

1 AuthorsBeguin P.
TitleMolecular biology of cellulose degradation.
SourceAnnu. Rev. Microbiol. 44:219-248(1990).
PubMed ID2252383
DOI10.1146/annurev.mi.44.100190.001251
2 AuthorsGilkes N.R., Henrissat B., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
TitleDomains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
SourceMicrobiol. Rev. 55:303-315(1991).
PubMed ID1886523
3 AuthorsHenrissat B., Bairoch A.
TitleNew families in the classification of glycosyl hydrolases based on amino acid sequence similarities.
SourceBiochem. J. 293:781-788(1993).
PubMed ID8352747
4 AuthorsDavies G.J., Dodson G.G., Hubbard R.E., Tolley S.P., Dauter Z., Wilson K.S., Hjort C., Mikkelsen J.M., Rasmussen G., Schuelein M.
TitleStructure and function of endoglucanase V.
SourceNature 365:362-364(1993).
PubMed ID8377830
DOI10.1038/365362a0
E1
Sourcehttp://www.expasy.org/cgi-bin/lists?glycosid.txt

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