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PROSITE documentation PDOC01046

MraY family signatures

Description:

Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) (mraY) [1] is a bacterial enzyme responsible for the formation of the first lipid intermediate of the cell wall peptidoglycan synthesis. It catalyzes the formation of undecaprenyl-pyrophosphoryl-N-acetylmuramoyl-pentapeptide from UDP-MurNAc-pentapeptide and undecaprenyl-phosphate.

It is an integral membrane protein with probably ten transmembrane domains. It belongs to family 4 of glycosyl transferases. Homologs of mraY have been found in the archaebacteria Methanobacterium thermoautotrophicum and in Arabidopsis thaliana.

We developed two patterns for this family of enzyme. The first one is located at the end of the first cytoplasmic loop and the beginning of the second transmembrane domain. The second pattern is located in the third cytoplasmic loop.

Expert(s) to contact by email:

Bouhss A.

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

MRAY_1, PS01347; MraY family signature 1  (PATTERN)
Consensus pattern: [KRA]-x(2)-[TIVK]-P-[ST]-[MGA]-[GA]-G-[LIVSA]-x-[LIVMF](2)
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01347
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01347
• Scan Swiss-Prot/TrEMBL entries against PS01347
• view ligand binding statistics
MRAY_2, PS01348; MraY family signature 2  (PATTERN)
Consensus pattern: [NHS]-x(2)-[NK]-x-[TINAS]-[DN]-G-[ILVM]-D-G-[LM]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: Haemophilus influenzae rfe, which also belongs to glycosyl transferase family 4.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01348
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01348
• Scan Swiss-Prot/TrEMBL entries against PS01348
• view ligand binding statistics

Reference:

1 AuthorsBouhss A., Mengin-Lecreulx D., Le Beller D., Van Heijenoort J.
TitleTopological analysis of the MraY protein catalysing the first membrane step of peptidoglycan synthesis.
SourceMol. Microbiol. 34:576-585(1999).
PubMed ID10564498

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