PROSITE documentation PDOC51643HD Cas3-type domain profile
Clustered regularly interspaced short palindromic repeats (CRISPRs) and Cas proteins represents an adaptative microbial immunity system against viruses and plasmids. Cas3 proteins have been proposed to play a key role in the CRISPR mechanism through the direct cleavage of invasive nucleic acids. Cas3 proteins are predicted to contain the DExD/H-box helicase (see <PDOC51192>) and HD domains, which can be covalently fused (Cas3) or encoded as individual proteins (Cas3' or Cas3'', respectively). The HD domain proteins represent a large group of enzymes, which catalyze phosphomonoesterase or phosphodiesterase reactions on a broad range of substrates including nucleotides and nucleic acids and function primarily in nucleic acid metabolism and signal transduction. The Cas3-type HD domain has nuclease activity against ssDNA and ssRNA [1,2].
The globular Cas3-type HD domain is composed of eigth α-helices and two short β-strands connected by extended loops (see <PDB:3S4L>) [2].
The profile we developed covers the entire Cas3-type HD domain.
Last update:April 2012 / First entry.
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PROSITE method (with tools and information) covered by this documentation:
| 1 | Authors | Sinkunas T. Gasiunas G. Fremaux C. Barrangou R. Horvath P. Siksnys V. |
| Title | Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system. | |
| Source | EMBO J. 30:1335-1342(2011). | |
| PubMed ID | 21343909 | |
| DOI | 10.1038/emboj.2011.41 |
| 2 | Authors | Beloglazova N. Petit P. Flick R. Brown G. Savchenko A. Yakunin A.F. |
| Title | Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference. | |
| Source | EMBO J. 30:4616-4627(2011). | |
| PubMed ID | 22009198 | |
| DOI | 10.1038/emboj.2011.377 |
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