To improve security and privacy, we are moving our web pages and services from HTTP to HTTPS.
To give users of web services time to transition to HTTPS, we will support separate HTTP and HTTPS services until the end of 2017.
From January 2018 most HTTP traffic will be automatically redirected to HTTPS. [more...]
View this page in https
PROSITE documentation PDOC00374 [for PROSITE entry PS00346]

Ets-domain signatures and profile





Description

Avian leukemia virus E26 is a replication defective retrovirus that induces a mixed erythroid/myeloid leukemia in chickens. E26 virus carries two distinct oncogenes, v-myb and v-ets. The ets portion of this oncogene is required for the induction of erythroblastosis. V-ets and c-ets-1, its cellular progenitor, have been shown [1] to be nuclear DNA-binding proteins. Ets-1 differs slightly from v-ets at its carboxy-terminal region. In most species where it has been sequenced, c-ets-1 exists in various isoforms generated by alternative splicing and differential phosphorylation.

A number of proteins, that share regions of similarity with v-ets/c-ets-1, have been found and are listed below:

  • Ets-2, which has been sequenced in mammals, chicken, Xenopus and Drosophila.
  • Human ELF-1, a transcription factor that appears to be required for the T- cell-receptor-mediated trans activation of HIV-2 genes.
  • Mammalian ELK-1, ELK-3 (also known as Net or SAP-2) and ELK-4 (also known as SRF accessory protein 1 (SAP-1)), which all form a ternary complex with the serum response factor (SRF).
  • Human ERF, a potent transcriptional repressor that binds to the HI element of the Ets-2 promoter.
  • Human ERG.
  • Human ERM.
  • Mammalian ETV1, a transcriptional activator.
  • FLI-1, a sequence specific transcriptional activator.
  • Adenovirus E1A enhancer-binding protein (E1A-F) (ETV4) (PEA3).
  • PU.1 (also known as Spi-1), a protein that binds to a purine-rich sequence, the PU-box, that can act as a lymphoid-specific enhancer. PU.1 is probably a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B cells. In mouse, Spi-1 is an oncogene involved in murine acute friend erythroleukemia.
  • Spi-B, a transcription factor that binds the PU-box.
  • GA binding protein (GAPB) α subunit. GAPB is a transcription factor capable of interacting with purine-rich repeats (GA repeats).
  • Drosophila protein elg-1.
  • Drosophila protein pokkuri (pok) (also known as yan), which is a negative regulator of photoreceptor development.
  • Drosophila protein pointed (pnt) which is required for glial-neuronal cell interactions.
  • Drosophila ecdysone induced protein 74E.

All these proteins contain a conserved domain, the 'ETS-domain', [2,3,4,5] involved in DNA-binding. It seems to recognize purine-rich sequences [5]. This domain, of about 85 to 90 amino acids, is rich in aromatic and positively charged residues. It is generally localized at the C-terminus of the protein, with the exception of ELF-1, ELK-1, ELK-3, ELK-4 and ERF where it is found at the N-terminus.

We have derived two signature patterns for the ETS-domain. The first one is based on a highly conserved region in the N-terminal part of the domain, while the second is based on a region in the second third of the domain. The profile spans the complete domain.

Last update:

April 2006 / Pattern revised.

Technical section

PROSITE methods (with tools and information) covered by this documentation:

ETS_DOMAIN_2, PS00346; Ets-domain signature 2  (PATTERN)

ETS_DOMAIN_3, PS50061; Ets-domain profile  (MATRIX)

ETS_DOMAIN_1, PS00345; Ets-domain signature 1  (PATTERN)


References

1AuthorsReddy E.S.P., Rao V.N.
TitleLocalization and modulation of the DNA-binding activity of the human c-ets-1 protooncogene.
SourceCancer Res. 50:5013-5016(1990).
PubMed ID2165853

2AuthorsKarim F.D., Urness L.D., Thummel C.S., Klemsz M.J., McKercher S.R., Celada A., Van Beveren C., Maki R.A., Gunther C.V., Nye J.A.
TitleThe ETS-domain: a new DNA-binding motif that recognizes a purine-rich core DNA sequence.
SourceGenes Dev. 4:1451-1453(1990).
PubMed ID2253872

3AuthorsMcLeod K., Leprince D., Stehelin D.
SourceTrends Biochem. Sci. 17:251-256(1992).

4AuthorsLaudet V., Niel C., Duterque-Coquillaud M., Leprince D., Stehelin D.
TitleEvolution of the ets gene family.
SourceBiochem. Biophys. Res. Commun. 190:8-14(1993).
PubMed ID8422262

5AuthorsWasylyk B., Hahn S.L., Giovane A.
TitleThe Ets family of transcription factors.
SourceEur. J. Biochem. 211:7-18(1993).
PubMed ID8425553

6AuthorsWang C.-Y., Petryniak B., Ho I.-C., Thompson C.B., Leiden J.M.
TitleEvolutionarily conserved Ets family members display distinct DNA binding specificities.
SourceJ. Exp. Med. 175:1391-1399(1992).
PubMed ID1569404



PROSITE is copyright. It is produced by the SIB Swiss Institute Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to
Prosite License or see: prosite_license.html.

Miscellaneous

View entry in original PROSITE document format
View entry in raw text format (no links)