PROSITE documentation PDOC00972
T-box domain signatures and profile


A number of eukaryotic DNA-binding proteins contain a domain of about 170 to 190 amino acids known as the T-box domain [1,2,3] and which probably binds DNA. The T-box has first been found in the mice T locus (Brachyury) protein, a transcription factor involved in mesoderm differentiation. It has since been found in the following proteins:

  • Vertebrate and invertebrate homologs of the T protein.
  • Mammalian proteins TBX1 to TBX20.
  • Mammalian protein TBR1 which is expressed specifically in brain.
  • Mammalian and Xenopus laevis eomesodermin (eomes or TBR2).
  • Xenopus laevis Vegt (or Antipodean), a transcription factor that activates the expression of wnt-8, eomes and Brachyury.
  • Chicken TbxT.
  • Drosophila protein optomotor-blind (omb).
  • Drosophila protein brachyenteron (byn) (also known as Trg), which is required for the specification of the hindgut and anal pads.
  • Drosophila protein H15.
  • Caenorhabditis elegans proteins tbx-1 to tbx-40.

As signature patterns for the T-domain, we selected two conserved regions. The first region corresponds to the N-terminal of the domain and the second one to the central part. A profile that spans the complete domain was also developed.

Last update:

December 2004 / Patterns and text revised.


Technical section

PROSITE methods (with tools and information) covered by this documentation:

TBOX_3, PS50252; T-box domain profile  (MATRIX)

TBOX_1, PS01283; T-box domain signature 1  (PATTERN)

TBOX_2, PS01264; T-box domain signature 2  (PATTERN)


1AuthorsBollag R.J. Siegfried Z. Cebra-Thomas J.A. Garvey N. Davison E.M. Silver L.M.
TitleAn ancient family of embryonically expressed mouse genes sharing a conserved protein motif with the T locus.
SourceNat. Genet. 7:383-389(1994).
PubMed ID7920656

2AuthorsAgulnik S.I. Garvey N. Hancock S. Ruvinsky I. Chapman D.L. Agulnik I. Bollag R. Papaioannou V. Silver L.M.
TitleEvolution of mouse T-box genes by tandem duplication and cluster dispersion.
SourceGenetics 144:249-254(1996).
PubMed ID8878690

3AuthorsPapaioannou V.E.
TitleT-box family reunion.
SourceTrends Genet. 13:212-213(1997).
PubMed ID9196325

PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see prosite_license.html.


View entry in original PROSITE document format
View entry in raw text format (no links)