We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
PROSITE documentation PDOC01036Pyrrolidone-carboxylate peptidase active sites
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PURL: https://purl.expasy.org/prosite/documentation/PDOC01036
Description
Pyrrolidone-carboxylate peptidase (EC 3.4.19.3) (PYRase) (also known as pyroglutamyl peptidase) is the enzyme that selectively removes pyroglutamate (pGlu) from the N-terminus of proteins and peptides.
In bacteria and archebacteria PYRase (gene pcp) is a protein of 22-25 kD. It is a cysteine protease with a Cys-His-Glu catalytic triad [1,2]. We developed two signature patterns that respectively include the glutamate and cysteine active site residues.
Note:These proteins belong to family C15 in the classification of peptidases [3,E1].
Last update:December 2004 / Pattern and text revised.
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Technical section
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References
| 1 | Authors | Singleton M.R. Isupov M. Littlechild J. |
| Title | X-ray structure of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis. | |
| Source | Structure 7:237-244(1999). | |
| PubMed ID | 10368293 |
| 2 | Authors | Odagaki Y. Hayashi A. Okada K. Hirotsu K. Kabashima T. Ito K. Yoshimoto T. Tsuru D. Sato M. Clardy J. |
| Title | The crystal structure of pyroglutamyl peptidase I from Bacillus amyloliquefaciens reveals a new structure for a cysteine protease. | |
| Source | Structure 7:399-411(1999). | |
| PubMed ID | 10196127 |
| 3 | Authors | Rawlings N.D. Barrett A.J. |
| Title | Families of cysteine peptidases. | |
| Source | Methods Enzymol. 244:461-486(1994). | |
| PubMed ID | 7845226 |
| E1 | Title | https://www.uniprot.org/docs/peptidas |
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