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We are deeply saddened by the passing of Amos Bairoch (1957–2025), the creator of PROSITE. We wish to dedicate our latest paper, published shortly before his death, to him. He will always be a source of inspiration to us.
Our deepest condolences go out to his family and friends, and to all those who had the privilege of working with him. Rest in peace, Amos. Your work will live on long after you are gone.
Amos Bairoch

PROSITE documentation PDOC51634
CRC domain profile


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PURL: https://purl.expasy.org/prosite/documentation/PDOC51634

Description

The following proteins of the tesmin/TSO1 family contain two cysteines-rich repeats with the consensus C-X-C-X(4)-C-X(3)-Y-C-X-C-X(6)-C-X(3)-C-X-C-X(2)-C separated by a region of variable length containing the short conserved sequence R-N-P-X-A-F-X-P-K:

  • Animal tesmin or MTL5, originally identified by its specific expression in testes, but subsequently it was also detected at specific stages of ovary development.
  • Animal tesmin-like (tesl) or LIN54.
  • Drosophila melanogaster tombola (tomb), a meiotic arrest protein which is expressed specifically in testis.
  • Arabidopsis thaliana TSO1, 'tso' means 'ugly' in Chinese and refers to the appearance of tso1 mutant flowers.
  • Arabidopsis thaliana TSO1-like 1 and 2 (SOL1 and SOL2).
  • Legume Cysteine-rich Polycomb-like Protein 1 (CPP1), a DNA-binding protein acting as a negative regulator of the leghemoglobin gene.

This domain has been named the CRC domain (C1-RNPXAFXPK-C2). It binds zinc and is able to bind DNA [1,2,3,4,5].

The CRC domain shows some similarity to the CXC domain found in the E(z)-type of Polycomb group proteins (see <PDOC51633>) [6]. However, a clear distinction can be made since the CXC domain lacks the RNPXAFXPK motif.

The profile we developed covers the entire CRC domain.

Last update:

January 2012 / First entry.

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Technical section

PROSITE method (with tools and information) covered by this documentation:

CRC, PS51634; CRC domain profile  (MATRIX)


References

1AuthorsSong J.-Y. Leung T. Ehler L.K. Wang C. Liu Z.
TitleRegulation of meristem organization and cell division by TSO1, an Arabidopsis gene with cysteine-rich repeats.
SourceDevelopment 127:2207-2217(2000).
PubMed ID10769244

2AuthorsHauser B.A. He J.Q. Park S.O. Gasser C.S.
TitleTSO1 is a novel protein that modulates cytokinesis and cell expansion in Arabidopsis.
SourceDevelopment 127:2219-2226(2000).
PubMed ID10769245

3AuthorsCvitanich C. Pallisgaard N. Nielsen K.A. Hansen A.C. Larsen K. Pihakaski-Maunsbach K. Marcker K.A. Jensen E.O.
TitleCPP1, a DNA-binding protein involved in the expression of a soybean leghemoglobin c3 gene.
SourceProc. Natl. Acad. Sci. U.S.A. 97:8163-8168(2000).
PubMed ID10859345
DOI10.1073/pnas.090468497

4AuthorsAndersen S.U. Algreen-Petersen R.G. Hoedl M. Jurkiewicz A. Cvitanich C. Braunschweig U. Schauser L. Oh S.-A. Twell D. Jensen E.O.
TitleThe conserved cysteine-rich domain of a tesmin/TSO1-like protein binds zinc in vitro and TSO1 is required for both male and female fertility in Arabidopsis thaliana.
SourceJ. Exp. Bot. 58:3657-3670(2007).
PubMed ID18057042
DOI10.1093/jxb/erm215

5AuthorsJiang J. Benson E. Bausek N. Doggett K. White-Cooper H.
TitleTombola, a tesmin/TSO1-family protein, regulates transcriptional activation in the Drosophila male germline and physically interacts with always early.
SourceDevelopment 134:1549-1559(2007).
PubMed ID17360778
DOI10.1242/dev.000521

6AuthorsOgawa M. Hiraoka Y. Taniguchi K. Aiso S.
TitleCloning and expression of a human/mouse Polycomb group gene, ENX-2/Enx-2.
SourceBiochim. Biophys. Acta 1395:151-158(1998).
PubMed ID9473645



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