PROSITE documentation PDOC51875Hypovirulence-associated virus (HAV) papain-like proteases p29 and p48 domains profiles
Hypoviruses are positive-strand RNA mycoviruses that attenuate virulence of their pathogenic fungal hosts [E1]. They employ a gene expression strategy that involves the autocatalytic processing of the N-terminal portion of encoded polyproteins by papain-like protease domains. The prototypic hypovirus CHV-1/EP713, responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus Cryphonectria parasitica, encodes two contiguous open reading frames (ORFs) designated ORF A and ORF B, which contain N-terminal proteases p29 and p48, respectively. Protease p29 functions as a suppressor of the RNA silencing defense response, while p48 is required for viral RNA replication [1,2,3,4].
The HAV papain-like protease p29 is a cysteine protease, which forms the peptidase family C7 [E2]. A Cys and a His catalytic residue are essential for autocatalytic cleavage at a glycine dipeptide clevage site located at the C-terminus of the domain [1,2,4].
The HAV papain-like protease p48 is a cysteine protease, which forms the peptidase family C8 [E3]. A Cys and a His catalytic residue are essential for autocatalytic cleavage between the cleavage site residues Gly and Ala located at the C-terminus of the domain [1,2,4].
The profiles we developed cover the entire HAV papain-like proteases p48 and p29 domains.
Last update:November 2018 / First entry.
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PROSITE methods (with tools and information) covered by this documentation:
| 1 | Authors | Shapira R. Nuss D.L. |
| Title | Gene expression by a hypovirulence-associated virus of the chestnut blight fungus involves two papain-like protease activities. Essential residues and cleavage site requirements for p48 autoproteolysis. | |
| Source | J. Biol. Chem. 266:19419-19425(1991). | |
| PubMed ID | 1918054 |
| 2 | Authors | Choi G.H. Pawlyk D.M. Nuss D.L. |
| Title | The autocatalytic protease p29 encoded by a hypovirulence-associated virus of the chestnut blight fungus resembles the potyvirus-encoded protease HC-Pro. | |
| Source | Virology 183:747-752(1991). | |
| PubMed ID | 1853573 |
| 3 | Authors | Deng F. Nuss D.L. |
| Title | Hypovirus papain-like protease p48 is required for initiation but not for maintenance of virus RNA propagation in the chestnut blight fungus Cryphonectria parasitica. | |
| Source | J. Virol. 82:6369-6378(2008). | |
| PubMed ID | 18448523 | |
| DOI | 10.1128/JVI.02638-07 |
| 4 | Authors | Jensen K.S. Nuss D.L. |
| Title | Mutagenesis of the catalytic and cleavage site residues of the hypovirus papain-like proteases p29 and p48 reveals alternative processing and contributions to optimal viral RNA accumulation. | |
| Source | J. Virol. 88:11946-11954(2014). | |
| PubMed ID | 25100848 | |
| DOI | 10.1128/JVI.01489-14 |
| E1 | Title | https://viralzone.expasy.org/594 |
| E2 | Title | https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=C7 |
| E3 | Title | https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=C8 |
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