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Phosphofructokinase (EC 2.7.1.11) (PFK) [1,2] is a key regulatory enzyme in the glycolytic pathway. It catalyzes the phosphorylation by ATP of fructose 6-phosphate to fructose 1,6-bisphosphate. In bacteria PFK is a tetramer of identical 36 Kd subunits. In mammals it is a tetramer of 80 Kd subunits. Each 80 Kd subunit consist of two homologous domains which are highly related to the bacterial 36 Kd subunits. In Human there are three, tissue-specific, types of PFK isozymes: PFKM (muscle), PFKL (liver), and PFKP (platelet). In yeast PFK is an octamer composed of four 100 Kd α chains (gene PFK1) and four 100 Kd β chains (gene PFK2); like the mammalian 80 Kd subunits, the yeast 100 Kd subunits are composed of two homologous domains.

As a signature pattern for PFK we selected a region that contains three basic residues involved in fructose-6-phosphate binding.

Escherichia coli has two phosphofructokinase isozymes which are encoded by genes pfkA (major) and pfkB (minor). The pfkB isozyme is not evolutionary related to other prokaryotic or eukaryotic PFK's (see <PDOC00504>).

April 2006 / Pattern revised.

PROSITE method (with tools and information) covered by this documentation:

PHOSPHOFRUCTOKINASE, PS00433; Phosphofructokinase signature  (PATTERN)

Consensus pattern: [RK]-x(4)-[GAS]-H-x-[QL]-[QR]-[GS]-[GF]-x(5)-[DE]-[RL] The R/K, the H and the Q/R are involved in fructose-6-P binding Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00433 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00433 • Scan Swiss-Prot/TrEMBL entries against PS00433 • view ligand binding statistics

Matching PDB structures: 1MTO 1PFK 1ZXX 2PFK ... [ALL]

1	Authors	Poorman R.A., Randolph A., Kemp R.G., Heinrikson R.L.
	Title	Evolution of phosphofructokinase--gene duplication and creation of new effector sites.
	Source	Nature 309:467-469(1984).
	PubMed ID	6233492

2	Authors	Heinisch J., Ritzel R.G., von Borstel R.C., Aguilera A., Rodicio R., Zimmermann F.K.
	Title	The phosphofructokinase genes of yeast evolved from two duplication events.
	Source	Gene 78:309-321(1989).
	PubMed ID	2528496

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