PROSITE documentation PDOC00336

Phosphofructokinase signature




Description

Phosphofructokinase (EC 2.7.1.11) (PFK) [1,2] is a key regulatory enzyme in the glycolytic pathway. It catalyzes the phosphorylation by ATP of fructose 6-phosphate to fructose 1,6-bisphosphate. In bacteria PFK is a tetramer of identical 36 Kd subunits. In mammals it is a tetramer of 80 Kd subunits. Each 80 Kd subunit consist of two homologous domains which are highly related to the bacterial 36 Kd subunits. In Human there are three, tissue-specific, types of PFK isozymes: PFKM (muscle), PFKL (liver), and PFKP (platelet). In yeast PFK is an octamer composed of four 100 Kd α chains (gene PFK1) and four 100 Kd β chains (gene PFK2); like the mammalian 80 Kd subunits, the yeast 100 Kd subunits are composed of two homologous domains.

As a signature pattern for PFK we selected a region that contains three basic residues involved in fructose-6-phosphate binding.

Note:

Escherichia coli has two phosphofructokinase isozymes which are encoded by genes pfkA (major) and pfkB (minor). The pfkB isozyme is not evolutionary related to other prokaryotic or eukaryotic PFK's (see <PDOC00504>).

Last update:

April 2006 / Pattern revised.

Technical section

PROSITE method (with tools and information) covered by this documentation:

PHOSPHOFRUCTOKINASE, PS00433; Phosphofructokinase signature  (PATTERN)


References

1AuthorsPoorman R.A., Randolph A., Kemp R.G., Heinrikson R.L.
TitleEvolution of phosphofructokinase--gene duplication and creation of new effector sites.
SourceNature 309:467-469(1984).
PubMed ID6233492

2AuthorsHeinisch J., Ritzel R.G., von Borstel R.C., Aguilera A., Rodicio R., Zimmermann F.K.
TitleThe phosphofructokinase genes of yeast evolved from two duplication events.
SourceGene 78:309-321(1989).
PubMed ID2528496



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