Glucoamylase (GA) (EC 220.127.116.11) is an enzyme that catalyzes the release of D-glucose from the non-reducing ends of starch and other oligo- or
polysaccharides. Extensive studies of fungal GA have shown [1,E1] that three
closely clustered acidic residues play a role in the catalytic mechanism of
GA. The region that includes these residues is also conserved in a recently
sequenced bacterial GA . We used this region as a signature pattern.
These proteins belong to family 15 in the classification of glycosyl
A GA from Schwanniomyces occidentalis is not a member of this family
but belongs to family 31 (see <PDOC00120>).
July 1998 / Text revised.
PROSITE method (with tools and information) covered by this documentation:
Sierks M.R., Ford C., Reilly P.J., Svensson B.
Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori.
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