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Glucoamylase (GA) (EC 3.2.1.3) is an enzyme that catalyzes the release of D-glucose from the non-reducing ends of starch and other oligo- or polysaccharides. Extensive studies of fungal GA have shown [1,E1] that three closely clustered acidic residues play a role in the catalytic mechanism of GA. The region that includes these residues is also conserved in a recently sequenced bacterial GA [2]. We used this region as a signature pattern.

These proteins belong to family 15 in the classification of glycosyl hydrolases [3,E1].

A GA from Schwanniomyces occidentalis is not a member of this family but belongs to family 31 (see <PDOC00120>).

July 1998 / Text revised.

PROSITE method (with tools and information) covered by this documentation:

GLUCOAMYLASE, PS00820; Glucoamylase active site region signature  (PATTERN)

Consensus pattern: [STN]-[GP]-x(1,2)-[DE]-x-W-E-E-x(2)-[GS] All D/E are active site residues Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: 1.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00820 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00820 • Scan Swiss-Prot/TrEMBL entries against PS00820 • view ligand binding statistics

Matching PDB structures: 1AGM 1AYX 1DOG 1GAH ... [ALL]

1	Authors	Sierks M.R., Ford C., Reilly P.J., Svensson B.
	Title	Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori.
	Source	Protein Eng. 3:193-198(1990).
	PubMed ID	1970434

2	Authors	Ohnishi H., Kitamura H., Minowa T., Sakai H., Ohta T.
	Title	Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme.
	Source	Eur. J. Biochem. 207:413-418(1992).
	PubMed ID	1633799

3	Authors	Henrissat B.
	Title	A classification of glycosyl hydrolases based on amino acid sequence similarities.
	Source	Biochem. J. 280:309-316(1991).
	PubMed ID	1747104

E1
Source	http://www.cazy.org/fam/GH15.html

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