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FAD-dependent glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) (GPD) catalyzes the conversion of glycerol-3-phosphate into dihydroxyacetone phosphate. In bacteria [1] it is associated with the utilization of glycerol coupled to respiration. In Escherichia coli, two isozymes are known: one expressed under anaerobic conditions (gene glpA) and one in aerobic conditions (gene glpD). In eukaryotes, a mitochondrial form of GPD participates in the glycerol phosphate shuttle in conjunction with an NAD-dependent cytoplasmic GPD (EC 1.1.1.8) [2,3].

These enzymes are proteins of about 60 to 70 Kd which contain a probable FAD-binding domain in their N-terminal extremity. The mammalian enzyme differs from the bacterial or yeast proteins by having an EF-hand calcium-binding region (See <PDOC00018>) in its C-terminal extremity.

We developed two signature patterns. One based on the first half of the FAD-binding domain and one which corresponds to a conserved region in the central part of these enzymes.

Brown L.J.

December 2004 / Pattern and text revised.

PROSITE methods (with tools and information) covered by this documentation:

FAD_G3PDH_1, PS00977; FAD-dependent glycerol-3-phosphate dehydrogenase signature 1  (PATTERN)

Consensus pattern: [IV]-G-G-G-x(2)-G-[STACV]-G-x-[AT]-x-[DQ]-x(3)-[RAS]-G Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00977 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00977 • Scan Swiss-Prot/TrEMBL entries against PS00977 • view ligand binding statistics

Matching PDB structures: 2QCU 2R45 2R46 2R4E ... [ALL]

FAD_G3PDH_2, PS00978; FAD-dependent glycerol-3-phosphate dehydrogenase signature 2  (PATTERN)

Consensus pattern: G-G-K-x(2)-[GSTE]-Y-R-x(2)-A Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00978 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00978 • Scan Swiss-Prot/TrEMBL entries against PS00978 • view ligand binding statistics

Matching PDB structures: 2QCU 2R45 2R46 2R4E ... [ALL]

1	Authors	Austin D., Larson T.J.
	Title	Nucleotide sequence of the glpD gene encoding aerobic sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12.
	Source	J. Bacteriol. 173:101-107(1991).
	PubMed ID	1987111

2	Authors	Roennow B., Kielland-Brandt M.C.
	Source	Yeast 9:1121-1130(1993).

3	Authors	Brown L.J., McDonald M.J., Lehn D.A., Moran S.M.
	Title	Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase cDNA. Evidence for EF-hand calcium-binding domains.
	Source	J. Biol. Chem. 269:14363-14366(1994).
	PubMed ID	8182039

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